Earticle

초록

영어

Cordycepin, a specific polyadenylation inhibitor, is the main functional component in Cordyceps militaris that is one of the top three renowned traditional Chinese medicines. In this study, we performed in vitro experiments to investigate the anti-invasive and anti-metastatic activities of cordycepin using human prostate carcinoma LNCaP cells.
Cordycepin were administered and their effects on LPS-induced cell migration and invasion by wound healing migration assay, measurement of TER and In vitro invasiveness assay. Within the concentrations which were not cytotoxic effects, cordycepin caused a concentration-dependent suppression of LPS-induced cell migration and invasion. The anti-invasive activity of cordycepin was also found to be associated with increased tightness of the TJ, which was confirmed by an increase in TER. The activity of MMP-2 in LNCaP cells was dose-dependently inhibited by treatment with cordycepin, and this was also correlated with a decrease in expression of its mRNA and proteins, and up-regulation of TIMPs expression. Additionally, cordycepin repressed the LPS-induced NF-kB activation and phosphorylation of PI3K/AKT. Taken together, these findings suggest that cordycepin inhibited LPS-induced migration and invasion of LNCaP cells by down-regulating the expression and activity of MMP-2, and the possible targets may be NF-kB and PI3K/AKT.

저자

• Jin-Woo Jeong [ Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735 ]
• Joon-Young Baek [ Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735 ]
• Cheng-Yun Jin [ Department of Biochemistry and Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan 614-052, Department of Biomaterial Control (BK21Program), Dongeui University Graduate School, Busan 614-714 ]
• Cheol Park [ Department of Biochemistry and Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan 614-052 ]
• Gi-Young Kim [ Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756 ]
• Yong-Kee Jeong [ Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714 ]
• Yung-Hyun Choi [ Department of Biochemistry and Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan 614-052, Department of Biomaterial Control (BK21Program), Dongeui University Graduate School, Busan 614-714, Blue-Bio Industry Regional Innovation Center, Dongeui University, Busan 614-714, Republic of Korea ]
• Jae-Dong Lee [ Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  버섯 [Mushroom]

• 간기

  반년간

• 수록기간

  1997~2025

• 십진분류

  KDC 525 DDC 635