rDNA-ITS 및 CAPS 분석에 의한 꽃송이버섯 (Sparassis crispa) 수집균주의 계통분류학적 특성구분
Phylogenetic relationships of medicinal mushroom Sparassis crispa strains using the rDNA-ITS and CAPS analysis
This study was carried out to analyze the genetic relationships among 22 strains of Sparassis crispa, which were collected from various regions of worldwide. The cleaved amplified polymorphic sequence were obtained from the ribosomal DNA ITS regions of each strain. Based on the sequence analysis, the presence of five different groups were observed. Most strains shared the high nucleotide sequence similarity (about 90%) to each other, except only one strain, KACC50866. Nucleotide sequence similarity of KACC50866 was below 10% to other strains, indicating the genetic relatedness of strain KACC50866 was low compared to other strains. More works such as mitochondria genome analysis should help to determine the precise genetic diversity of S. crispa strains.
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ABSTRACT 서론 재료 및 방법 공시균주 분자생물학적 유연관계 분석 제한효소 절단 분석 PCR 산물의 클로닝 및 염기서열 분석 결과 및 고찰 꽃송이버섯 수집균주의 ITS 영역 구조 분석 제한효소 절단 분석 ITS 영역의 염기서열을 이용한 꽃송이버섯속균의 유연관계 적요 인용문헌