Earticle

현재 위치 Home

포스터발표 - Ⅰ. 재배, 생리 활성

Production system of recombinant protein hIL-32 in Pleurotus eryngii using Agrobacterium-mediated transformation

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    한국버섯학회지 바로가기
  • 통권
    제7권 제2호 (2009.06)바로가기
  • 페이지
    pp.63-63
  • 저자
    Se-Eun Park, Seung Kim, Bong-Suk Choi, Hoe-Chang Kim, Sang-Jun Chung, Jun-Hui Choi, Sung-Jun Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A193708

원문정보

초록

영어
Human recombinant IL-32 induces the production of large amounts of several proinflammatory cytokines and chemokines, even in macrophage cell lines by activation of NF-κB and MAPK. The ability of IL-32 to potentiate inflammation is not the sole action of this cytokine. IL- 32 stimulates prostaglandin (PG)E2 in human PBMCs, which are pivotal in inflammation. The development of recombinant protein consisting of IL-32 is of great medical and industrial importance. Agrobacterium strains GV3101 harboring pCAMBIA1304 include IL- 32 vectors were used to infect the mushroom fruiting body pieces. For transformation experiments, fruiting body gill tissue pieces were vacuum infiltrated with the suspension of induced bacteria until the air had been completely purged. Mushroom mycelium appeared at the margins of the tissue pieces after 9 to 14 days on selection medium with hygromycin at 50㎍/㎖. Transformants PCR analyses confirmed that the IL-32 gene was into the genome of Pleurotus eryngii. The present results demonstrating the usefulness of the transgenic technique s in the genetic manipulation and improvement of mushroom.

저자

  • Se-Eun Park [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ] Corresponding author
  • Seung Kim [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
  • Bong-Suk Choi [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
  • Hoe-Chang Kim [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
  • Sang-Jun Chung [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
  • Jun-Hui Choi [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
  • Sung-Jun Kim [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    한국버섯학회지 [Journal of MUSHROOMS]
  • 간기
    계간
  • pISSN
    1738-0294
  • eISSN
    2288-8853
  • 수록기간
    2003~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 525 DDC 635

이 권호 내 다른 논문 / 한국버섯학회지 제7권 제2호

    피인용수 : 0(자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      페이지 저장