Earticle

초록

영어

Overproduction of reactive oxygen species (ROS) induces malfunctioning of the cell, thus leading to a number of pathological conditions such as oxidative-stress related disorders. A well- defined example of cellular damage is lipid peroxidation. Malondialdehyde (MDA) is a stable by-product of lipid peroxidation which easily combines with thiobarbituric acid reactive substances (TBARS) giving a fluorescent red derivative that can assayed spectrophotometrically. On the basis that urinary MDA can be a direct indicator of oxidative stress, we measured urinary MDA using recently developed urinary MDA analyzer and TBARS kit respectively, and studied correlations between two methods on 27 healthy volunteers. As a result highly accurate and reproducible results were obtained from the analyzer with the mean concentration of 451.20 ± 3.35 free radical point (FP) equivalent to 43.95 % normalized data. The detected values was correlated to TBARS kit method with the mean value of 6.632 ± 0.956 (36.60% normalized data) and the correlation value shows positive association with medium strength, r = 0.3126. The Urinary MDA analyzer works on same principle with colorimetric detection of MDA using TBARS, however the analyzer has short analysis time and the possible artifactual formation of MDA during heating with colorimetric TBARS method is abrogated. This is the first analyzer introduced that can detect urinary MDA, clinically implying its effectiveness and specificity in screening and monitoring oxidative stress-related diseases.

목차

ABSTRACT
 Introduction
 Materials and Methods
  1. Study subjects
  2. MDA Quantitation Using the Urine-MDA analyzer
  3. Preparation of Reagents for TBARS Assay
  4. MDA Quantitation Using TBARS Assay
  5. Statistical analysis
 Results and Discussion
 References

키워드

저자

• Rosa Mistica Ignacio [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University ]
• Kyung-Bok Joo [ Department of Ophthalmic Optics, Chodang University ]
• Easter Joy Sajo [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University ]
• Dong-Heui Kim [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University ]
• Kyu-Jae Lee [ Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University ] Correspondence

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국물학회지 [Korean Journal of Waters]

• 간기

  부정기

• pISSN

  2233-4653

• 수록기간

  2010~2021

• 십진분류

  KDC 539 DDC 628