Earticle

초록

영어

Reynoutria elliptica is Asian as traditional herbal medication. In this study, we examined the Inhibition of melanogensis and anti-UV properties ethanol extract of R. elliptica. Total polyphenols and flavonoids contents of R. elliptica, the result was respectively 209.2 ±1.2, 83.4±2.0 mg/g. Electron-donating ability of ethanol extract of R. elliptica was in 400 ㎍/㎖ as 92.92±1.51%. Tyrosinase have suppress effect in a dose dependent. UV-blocking effect was also showed a strong UV absorption in the range of UV-A(350-370 ㎚). In addition we tested tyrosinase inhibition activity and melanin contents on SK-MEL-2. Tyrosinase was inhibited to 40% at the dose of 20 ㎍/㎖ and melanogenesis was inhibited was 30%. Overall, our results indicate that R.elliptica extract showed that the α-MSH-mediated upregulation of tyrosinase activity and melanogenesis were inhibited in the extract does dependent manner in Sk-MEL-2 cells. Hence, R. elliptica extract could be applied as a type of dermatological whitening and anti-UV agent in skin care products.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 재료 및 시료의 추출
  2. 총 폴리페놀 함량 측정
  3. 총 플라보노이드 함량 측정
  4. Free radical 소거활성
  5. in vitro tyrosinase 저해활성 측정
  6. 자외선 흡수량 측정
  7. 세포주 및 세포 배양
  8. 세포생존율 측정
  9. SK-MEL-2세포에서 Tyrosinase 저해활성 측정
  10. SK-MEL-2 세포에서 Melanin 생합성 저해율 측정
  11. α-MSH 유도에 의한 Tyrosinase 활성도 및 Melanin 생성량에 미치는 영향 측정
  12. 통계분석
 Ⅲ. 연구결과 및 고찰
  1. 호장근 추출물의 총 폴리페놀 및 플라보노이드 함량
  2. 호장근 추출물의 항산화 활성
  3. 호장근 추출물의 in vitro tyrosinase 저해 활성
  4. 호장근 추출물의 UV 스펙트럼
  5. 호장근 추출물의 SK-MEL-2 세포 생존율
  6. SK-MEL-2 세포내의 Tyrosinase 활성도 및 Melanin 생성량에 미치는 영향
  7. 호장근 추출물이 α-MSH 유도에 의한 Tyrosinase 활성도 및 Melanin 생성량에 미치는 영향
 Ⅴ. 결론
 참고문헌

키워드

저자

• 김민정 [ Min-jung Kim | 신흥대학교 뷰티아트디자인과 ]
• 유민정 [ Min-Jeong Ryu | 영남이공대학교 박승철헤어과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646