Earticle

초록

영어

The aim of this study was to investigate whitening effect of 4- hydroxy-2 (or 5)-ethyl-5 (or 2)-methyl-3 (2H)-furanone (HEMF). The antioxidant activity of HEMF was evaluated using in vitro 1,1- diphenyl-2-picrylhydrazy (DPPH) free radical assay. Inhibitory effects of HEMF against tyrosinase activity and melanogenesis in B16 melanoma cells were assessed in vitro to determine whitening effect. HEMF showed strong radical scavenging activity in DPPH assay. The SC50 (concentration that inhibits 50% of the DPPH radical) values of HEMF was 1.5 mg/L. 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay with B16 melanoma cells showed that HEMF was not toxic up to the concentration of 1000 mg/L. HEMF induced dose-dependent inhibition of mushroom tyrosinase activity showing 71% inhibition at a concentration of 250 mg/L. Various concentrations (2.5-50 mg/L) of HEMF also significantly inhibited tyrosinase activity in B16 melanoma cells by 20-65 %. In addition, HEMF significantly reduced melanin contents of B16 melanoma cells by 52 % at a concentration of 50 mg/L. These results suggest that HEMF could be an effective whitening agent.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 시료 및 기기
  2. 세포주 및 세포배양
  3. 자유 라디컬 소거력 측정
  4. 세포 생존력 측정
  5. Mushroom tyrosinase 활성 억제 실험
  6. B16 세포에서 tyrosinase 활성 억제 실험
  7. B16 세포에서 melanin 생성 억제 효과 실험
  8. 통계처리
 Ⅲ. 연구결과 및 고찰
  1. 자유라디컬 소거력 측정
  2. 세포 생존력 측정
  3. Mushroom tyrosinase 활성 억제 실험 결과
  4. B16 melanoma세포에서 tyrosinase 활성 억제 실험 결과
  5. B16 melanoma 세포에서 멜라닌 생성 억제 효과 실험 결과
 Ⅳ. 결론
 감사의 글
 참고문헌

키워드

저자

• 장혜인 [ Hye-In Jang | 경기대학교 대체의학대학원 ] Corresponding author
• 고혜정 [ Hye-Jung Koh | 수원여자대학교 미용예술학과 ]
• 한영숙 [ Young-Sook Han | 동남보건대학 피부미용과 ]
• 김재희 [ Jaehee Kim | 경기대학교 대체의학대학원 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646