Earticle

초록

영어

Quercetin is a polyflavonoid used at the raw material of foods, cosmetics, medicines as an anti-oxidant, antiallergic, anti-tumor and anti-inflammatory agent. However, its intracellular mechanisms and signaling pathways on skin cells are not fully understood. In this study, we investigated the molecular mechanisms of quercetin in human dermal fibroblasts (HDFs). To determine cell viability on quercetin, we performed MTT assay in quercetin-treated HDFs. Quercetin did not cause any significant changes in viability of HDFs. Quercetin induced accumulation of p53 and phosphorylation of p38 MAPK. Cell cycle analysis using flow cytometry in quercetin-treated HDFs determined that quercetin caused G2/M phase arrest in a dose-dependent manner. DNA microarray analysis revealed that expression of various genes were regulated in quercetin-treated HDFs, and several transcripts showing critical levels of changes in their expression were validated by qRT-PCR. Quercetin induced the expression of HO-1, E2F7, TP53I3, known to be associated with cell protection, in doseand time-dependent manners. Taken together, these results demonstrate that quercetin may be used as an effective cosmeceutical material useful for protection of the dermal skin.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 세포배양
  2. 세포 생존율 측정
  3. RNA 분리 및 qRT-PCR
  4. Microarray
  5. Western blotting
  6. Flow cytometry
 Ⅲ. 연구결과 및 고찰
  1. 쿼세틴에 의한 세포 생존율 확인
  2. 쿼세틴에 의한 단백질 발현 변화
  3. 쿼세틴에 의한 G2/M arrest의 증가
  4. 쿼세틴 특이적인 유전자 발현 변화
  5. qRT-PCR을 통한 유전자 발현 검증
 Ⅳ. 결론
 감사의 글
 참고문헌

키워드

저자

• 홍미선 [ Mi Seon Hong | 건국대학교 질병분자표적신약연구센터 ]
• 윤영민 [ Yeongmin Yoon | 건국대학교 질병분자표적신약연구센터 ]
• 안성관 [ Sungkwan An | 건국대학교 질병분자표적신약연구센터 ]
• 안인숙 [ In-Sook An | 한국피부임상과학연구소 ]
• 변부형 [ Boohyeong Byun | 대구한의대학교 한의예과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646