To evaluate the effect of Rosemary(Rosemarinus officinalis) extract on antioxidative activity and melanogenesis on cultured SK-MEL-3, the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, tyrosinase activity and a total amount of melanin were measured. In this study, SK-MEL-3 cells were treated with the media containing 1∼20μM, hydrogen peroxide(H2O2) for 4 hours. H2O2 significantly decreased cell viability compared with control and XTT50 value was determined at 20μM of H2O2. In the antioxidative effect of Rosemary extract, it showed DPPH-radical scavenging activity and SOD-like activity. While, in the melanogenic effect of Rosemary extract, it significantly decreased the tyrosinase activity and a total amount of melanin which were increased by H2O2. From above the results, it is suggested that Rosemary extract was effective in the protection of H2O2-induced cytotoxicity by antioxidative activity and inhibition of melanogenesis.
목차
Abstract I. 서론 II. 연구방법 1. 실험재료 2. 실험방법 3. 통계처리 III. 결과 및 고찰 1. 세포생존율 측정 2. H2O2에 대한 로즈마리 추출물의 영향 3. DPPH-자유기 소거능(DPPH-radical scavenging activity) 측정 4. SOD-유사활성 (SOD-like activity) 측정 5. 티로시나제활성 (tyrosinase activiy) 측정 6. 총멜라닌량 측정 IV. 결론 참고문헌