Earticle

초록

영어

This study was to investigate the ethanol extracts of Morus alba on tyrosinase activity and melanogenesis. In the present study , we examined the effects of Morus alba ethanol extract on mushroom tyrosinase activity in vitro, on SK-MEL-2 cell tyrosinase activity , melanin contents, expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein1(TRP-1) and and tyrosinase-related protein2(TRP-2). Mushroom tyrosinase activity of dose dependant and about 75% at concentration of 400 ㎍/㎖. Morus alba ethanol extracts reduced melanin contents of SK-MEL-2 melanoma cella in a dose dependant manner and decreased to about 83.2% at a cocentration of 100 ㎍/㎖ without cell cytotoxicity(below 100 ㎍/㎖) and intracellular tyrosinase activity about 54.2% at concentration of 100 ㎍/㎖. Morus alba ethanol extracts inhibited tyrosinase and TRP-2 expression at protein level. These results suggest that Morus alba ethanol extracts reduced melanin formation by the inhibitied of tyrosinase activity and expression in SK-MEL-2 melanoma cells. Therefor, we confirmation that Morus alba ethanol extracts could be used as a useful whitening agent.

목차

Abstract
 I. 서론
 II. 재료 및 방법
  1. 재료 및 시료의 추출
  2. 세포주 및 세포 배양
  3. in vitro tyrosinase 저해활성 측정
  4. 세포생존율 측정
  5. SK-MEL-2세포에서 Tyrosinase 저해활성 측정
  6. SK-MEL-2세포에서 Melanin 생합성 저해율 측정
  7. a-MSH 유도에 의한 Tyrosinase 활성도 및 Melanin 생성료때| 미치는 영향 측정
  8. Western blot 분석
  9. 통계분석
 III. 결과
  1. in vitro tyrosinase 저해 활성
  2. SK-MEL-2 세포 생존율
  3. SK-MEL-2 세포내의 Tyrosinase 활성도 및 Melanin 생성량 미치는 영향
  4. 추출물이 Melanin 합성 관련 단백질 발현에 미치는 영향
  5. a-MSH 유도에 의한 Tyrosinase 활성도 및 Melanin 생성량에 미치는 영향
  6. α-MSH의 과 색소침착 시 Melanin 합성 관련 단백질 발현에 미치는 추출물의 영향
 V. 고찰
 VI. 결론
 참고문헌

키워드

저자

• 유민정 [ Ryu, Min-Jeong | 영남이공대학교 박승철헤어과 ] 교신담당저자

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한피부미용학회지 [Korean Journal of Aesthetics and Cosmetology]

• 간기

  격월간

• pISSN

  1738-4567

• 수록기간

  2003~2015

• 십진분류

  KDC 593 DDC 646