Earticle

초록

영어

In order to enhance the selectivity, productivity and yield of methyl fructoside, which was synthesized by enzymatic glycosylation of sucrose and methanol solution, controlling of surface property of solid support using different immobilization procedures optimized microenvironment of immobilized invertase. Silanization and polyethylene imine coating methods were adopted to give a hydrophobic and hydrophilic environment of immobilized invertase. As a result, polyethyleneimine coating method gave higher loading of enzyme, effective activity, and relative activity than silanization method, because it brought on increasing the functional density of amino group and enhancing the conservation of activity by regulating of hydrophilicity. And then, hydrophilic environment was possible to restraint the assessing of methyl fructoside molecule, which was more hydrophobic than sucrose, fructose, and glucose molecule in the reaction mixture, into .the active site of immobilizedinvertase. Consequently, hydrophilic microenvironment of immobilized invertase by polyethyleneimine coating obtained higher yield and productivity with increasing conversion than silanized and native invertase. Thus, this procedure optimized the microenvironment of immobilized invertase suitable for the enzymatic synthesis of methyl fructoside.

목차

Abstract
 1. 서론
 2. 이론적 배경
  2.1 메틸 프럭토시드의 합성반응
 3. 재료 및 방법
  3.1 전화당 효소의 고정화
  3.2 메틸 프럭토시드 생산성에 대한 담체 표면특성의 영향
  3.3 최적 고정화 담체의 선정
 4. 결과 및 고찰
  4.1 고정화 효소의 유효 활성도와 상대 활성도에 대한 담체 표면특성의 영향
  4.2 메틸 프럭토시드 생산성에 대한 담체 표면특성의 영향
  4.3 담체표면 특성에 따른 고정화 전화당 효소의 Vm과 Km 비교
 5. 결론
 참고문헌

저자

• 허주형 [ Heo, Joo Hyung | 서강대학교 화학공학과 ]
• 안형환 [ An, Hyung Hwan | 충주대학교 안전공학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  대한안전경영과학회지 [Journal of Korea Safety Management & Science]

• 간기

  계간

• pISSN

  1229-6783

• eISSN

  2288-1484

• 수록기간

  1999~2025

• 십진분류

  KDC 530 DDC 620