Earticle

초록

영어

Staphylokinase, a 163 amino aci d bacterial protein from Staphylococcus aureus is reported to have a therapeutic function and used as a potential alternative for the available clot dissolving drugs and some countries proved to be a potent alternative over the available clot dissolving drugs. In the present study, sak gene is isolated from a lysogenically converted S.aureus and expressed in non-pathogenic E.coli expression host. The sak gene is cloned into pET-28a vector and expressed in an IPTG and salt induced E. coli strain (GJ1158). The mature recombinant sak protein expressed in E.coli is also further extracted and analyzed by various methods to demonstrate thrombolytic activity.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1 Microbial Stains
  2.2 Culture Conditions
  2.3 Isolation and Screening of Staphylokinase Producing Microbial Source
  2.4 Identification and Cloning of Sak Gene from Isolated Sample
  2.5 Expression and Activity Analysis of Recombinant SAK
 3. Results and Discussion
  3.1 Identification of Collected Sample
  3.2 Screening for Staphylokinase Activity
  3.3 Isolation of Gene Coding for SAK
  3.4 Generation of Recombinant DNA
  3.5 Expression and Activity Analysis
 4. Conclusion
 References

키워드

저자

• KK Pulicherla [ Department of Biotechnology, R.V.R & J.C. College of Engineering ]
• GS Gadupudi [ University of Memphis Department of Biological sciences ]
• VPB Rekha [ Department of Biotechnology, R.V.R & J.C. College of Engineering ]
• K Seetharam [ Department of Biotechnology, Acharya Nagarjuna University ]
• Anmol Kumar [ Department of Biotechnology, Acharya Nagarjuna University ]
• KRS Sambasiva Rao [ Department of Biotechnology, Acharya Nagarjuna University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Advanced Science and Technology

• 간기

  월간

• pISSN

  2005-4238

• 수록기간

  2008~2016

• 십진분류

  KDC 505 DDC 605