Earticle

초록

영어

We examined the in vitro mutagenic and antimutagenic effects of hexane- extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities <400 g/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.

목차

Abstract
 서론
 재료 및 방법
  재료
  삼종실유
  Gas chromatography-mass spectrometry analysis
  총 플라보노이드 및 총 페놀화합물 함량측정
  조직세포 배양
  MTT assay
  간조직 대사 활성제
  Salmonella 돌연변이능 테스트
  Salmonella 항돌연변이 테스트
  통계 분석
 결과 및 고찰
  삼종실유의 지방산 함량
  MTT assay
  Ames test
 감사의 글
 문헌

키워드

저자

• 전정애 [ Jungae Jeun | 고려대학교 식품공학부 ]
• 조희준 [ Hee Joon Cho | 고려대학교 식품공학부 ]
• 전희진 [ Hee-jin Jun | 고려대학교 식품공학부 ]
• 이지혜 [ Ji Hae Lee | 고려대학교 식품공학부 ]
• 가요요 [ Yaoyao Jia | 고려대학교 식품공학부 ]
• 조경상 [ Kyoung Sang Cho | 건국대학교 생명과학과 ]
• 김은수 [ Eun Soo Kim | 건국대학교 생명과학과 ]
• 이성준 [ Sung-Joon Lee | 고려대학교 식품공학부 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국식품과학회지 [Korean Journal of Food Science and Technology]

• 간기

  격월간

• pISSN

  0367-6293

• 수록기간

  1969~2011

• 십진분류

  KDC 574 DDC 664