Fractional solvent extraction by organic solvents such as hexane, chloroform, ethylacetate, and butanol was carried out using 70% ethanol extract of apple flower leaves. Biological activities including antioxidant, whitening, antimicrobial and anti-wrinkle activities were investigated and bio-active materials of the extracts were identified using GC/MSD. Among the tested solvent fractions, ethylacetate fraction showed the highest total polyphenol content (1218.94 μg/mL), and flavonoid (140 μg/mL). The DPPH radical scavenging activities was over 80% at a dry matterbased concentration of 200 μg/μL and SOD-like activity was over 90% at 50 μg/mL concentration in ethylacetate fraction that was slightly lower than of ascorbic aicd. Tyrosinase inhibition activity related to skin-whitening was over 60% by ethylacetate fraction of 100 μg/mL. As an anti-aging effect, elastase inhibitory activity was about 45% in ethylacetate fraction. Also, it showed a significantly antimicrobial activity against P. acenes. From GC/MSD analysis, a characteristic peak of high content in ethylacetate fraction was identified as kaempferol, which has been reported as a bioactive compound.
목차
Abstract 1. 서론 2. 재료 및 방법 2.1. 재료 및 기기 2.2. 사과꽃잎 용매 분획별 추출 2.3. 총 폴리페놀 함량 2.4. 총 플라보노이드 함량 2.5. DPPH radical 소거능 측정 2.6. SOD (superoxide dismutase) 유사활성 측정 2.7. 미백활성 (Tyrosinase inhibition assay) 2.8. Elastase 저해활성 2.9. 항균활성 2.10. GC/MSDD 분석 3. 결과 및 고찰 3.1. 분획별 사과꽃잎의 추출 수율 3.2. 총 폴리페놀 함량 3.3. 총 플라보노이드 함량 3.4. DPPH radical 소거능 측정 3.5. SOD (superoxide dismutase) 유사활성 측정 3.6. 미백활성 (Tyrosinase inhibition assay) 3.7. Elastase 저해활성 3.8. 항균 활성 3.9. GC/MSD 분석 References
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동