Earticle

초록

영어

The growth behavior of recombinant Escherichia coli cells having plasmid pIF-III-B, which carries human alpha-interferon gene under the control of lpp promoter, lac promoter and lac operator, was studied by using of various E. coli host strains. Expression of the alpha-IFN gene is controllable by using inducer IPTG because the plasmid also contains lacI gene which produces lac regressors. The repressors block the transcription of alpha-IFN gene. There were considerable differences in cell growth according to the host strains used. Cell growth was inhibited not only by plasmid pIF-III-B itself but also by the induction of alph-a-IFN gene expression. Growth inhibition caused by the plasmid itself was more serious than that caused by the induction of alpha-IFN gene expression.

한국어

대장균의 lipoprotein promoter, lactose promotor 및 operator 와 lipoprotein의 signal sequence 를 가지는 vector에alpha-IFN 유전자가 cloning된 plasmid pIF-Ill-B를 여러종류의 대장균 숙주 세포에 형칠전환하여 alpha-IFN 의 생산성, 생장특성을 조사하였다. 또한 plasmid 자체와 cloning된 alpha-IFN 유전자의 발현유도가 세포생장에 미치는 영 향을 조사하였다.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Bacterial strains and Plasmid and culture conditions
  Induction of gene expression
  Transformations
  Preparation of recombinant cell extract for the assay of alpha-lFN
  Determination of alpha-lFN activity
 RESULTS
  Growth behavior of various E. coli strains harboring Plasmids
  Cloned alpha-lFN gene Product formation
  Effect of Plasmid PIF- III-B and induction of gone expression on cell growth
 DISCUSSION
 ABBREVIATIONS
 요약
 REFERENCES

저자

• Kap-Soo Noh [ 노갑수 | 서울대학교 공과대학 공업화학과 ]
• Cha-Yong Choi [ 최차용 | 서울대학교 공과대학 공업화학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576