Earticle

It has been reported that singe-strand (ss) DNA breaks could be photosensitized by some drugs. However, conventional methods to monitor the genotoxicity of the photosensitizing drugs are limited to the application for a high throughput screening of the photo-toxic drug because they are time consuming for the biological or chemical modification of the tested DNA and instrument-oriented. Therefore, We have investigated a colorimetric method to visualize ss DNA breaks using gold nanoparticles (AuNPs). ss DNA is known to physically adsorb onto AuNPs and prevent them from salt-induced aggregation. However, AuNPs mixed with UV irradiated ss DNA in the presence of photosensitizing drugs were aggregated after the salt addition into the mixture, resulting in red-to-purple color change. The extent of AuNP-aggregation was dependent on the UV-irradiation time and concentration of photosensitizers. In current, we are investigating the principle for the aggregation of AuNPs by only the ss DNA which were irradiated by UV light in the presence of ss DNA break photosensitizing drugs. The detail experimental results and working principle will be presented.

As an example of many possible applications of OSMI data, we present a method to detect red tides. In Korean waters, red tides usually occur in the South Sea where the turbidity is usually high due to strong tidal mixing in the shallow sea. The conventional case 1 chlorophyll algorithm cannot be applied since it cannot distinguish chlorophyll from SS (suspended sediments). In October 1998, a red tide outbreak occurred off the coast of Kunsan. We analyzed the SeaWiFS data of the outbreak. The standard SeaWiFS chlorophyll algorithm OC2 was poor in identifying the red tides. However, comparison of spectra of normalized water-leaving radiance indicates that red tide pixels can be distinguished from sediment-laden pixels. Channel 443 and 555 were effective in showing the spectral characteristics. We suggest K490 algorithm as an example in summarizing the information of the spectra and thereby in distinguishing the red tide pixels. Further development is desirable.

As an example of many possible applications of OSMI data, we present a method to detect red tides. In Korean waters, red tides usually occur in the South Sea where the turbidity is usually high due to strong tidal mixing in the shallow sea. The conventional case 1 chlorophyll algorithm cannot be applied since it cannot distinguish chlorophyll from SS (suspended sediments). In October 1998, a red tide outbreak occurred off the coast of KunSan. We analyzed the SeaWiFS data of the outbreak. The standard SeaWiFS chlorophyll algorithm OC-2 was poor in identifying the red tides. However, comparison of spectra of normalized water-leaving radiance indicates that red tide pixels can be distinguished from sediment-laden pixels. Channel 443 and 555 were effective in showing the spectral characteristics. We suggest K490 algorithm as an example in summarizing the information of the spectra and thereby in distinguishing the red tide pixels. Further development is desirable.

The space and ground based infrared spectra of red supergiants are modeled and arranged in order of their evolutionary status with their theoretical model Parameters. Because of their large amplitude pulsation, the observational data taken at different phases show wide discrepancies. The chemical compositions of the dust shells around red supergiants are affected by the nuclearreactions and dredge-up processes of the cental stars. Those processes aresensitiTelr dependent on the initial ma:ss, the initial chemical coMposition,and the evolutionarr status. Miras, infrared carbon stars, and OHAR starshaTe a close link in their evolution iii many aspects, i.e. the chemicalcomposition, the optical depths and the mass loss rates. The evolutionarytracks for the three classes of red super91iants on infrared Huo-color diagamhave been constructed.

To carry out baseline studies on monitoring systems for red tides in Jinhae bay, measurements and analyses were made on seawater samples from 15 sampling stations during 15 months from July, 1979. Water quality parameters studied are temperature, pH, DO, salinity, COD, SS, NO$\sub$3/, NO$\sub$2/, PO$\sub$4/, SiO$\sub$2/, Ca, Mg, Cd, Cu, Pb, Zn, Chlorophyll ${\alpha}$, diatoms and dinoflagellates. Multiple regression analyses were undertaken with chlorophyll ${\alpha}$, cell numbers of diatoms and dinoflagellates as the dependent variables and water quality parameters as the independent variables. The results showed that biomass, expressed as total cell numbers of diatoms and dinoflagellates, was largely influenced by COD, salinity and nutrients.

중년 이후의 여성의 경우 칼슘 섭취가 부족하거나, 인위적인 난소 적출 또는 폐경 등으로 인하여 골밀도가 감소하는 경향이 있다. 이 경우 교정 치료 시, 골내 고정원으로 마이크로스크류를 이용할 수 있는지를 알아보기 위하여, 생후 4개월 된 Sprague-Dawley계 백서를 실험군으로 난소 적출(Ovariectomy Screw, OS)군, 대조군으로 Sham operation 시행한(Sham operation Screw, SS) 군의 두 군으로 나누었다. OS군과 SS군 모두 상악 대구치 사이 구개골에 마이크로스크류를 식립하였고, NiTi coil spring을 이용하여, 상악 중절치 2개를 견인하였다. 각 군당 3일, 7일 후 7마리씩 희생시켰다. 희생 3일전 Alizarin red S를 주입하고, 상악골, 장골, 심장혈을 채득한 후, 심장혈을 성분분석하고, 상악골과 장골은 비탈회 레진표본을 제작하여, 편광현미경, 형광현미경으로 관찰하였다. 심장혈을 통한 성분 조사 결과 OS군, SS군 모두 7일째 alkaline phosphatase (ALP)의 농도가 높게 나타나 골개조의 가능성을 보였고, 편광현미경 사진에서 SO, SS군에서 모두 마이크로스크류의 압박측, 긴장측, 치아의 긴장측에서 3일 보다 7일에서 기존골에 비하여 골밀도가 낮은 영역이 적었고, 치아의 압박측에서는 골밀도가 낮은 영역이 증가하였다. 형광 현미경 관찰결과는 3일째 보다 7일째에 더 Alizarin red S로 침착된 골이 나타난 것으로 보아, 새로운 골의 형성이 있었음을 나타내었다. 골다공증이 유도된 백서에서 계속된 골의 흡수보다는 골개조의 가능성이 증가하고, 신생골의 형성이 증가함을 확인할 수 있었고, 이는 골다공증에서도 마이크로스크류의 식립으로 고정원 확보가 가능한 것으로 생각한다.

Most elderly women experience a decrease in their bone density due to a deficiency of calcium intake, ovariectomy, or menopause. This study evaluated the usability of the microscrew as a skeletal anchorage system in these orthodontic treatment cases, using rats as a research group. The 4 month old sprague-dawley species rats were divided into two groups, the OS (Ovariectomy Screw), and the SS (Sham operation Screw) group. In both the OS and SS groups, microscrews were implanted into the palatal bone between the upper molar teeth and two upper incisors were retracted using NETE coil spring with 75 g of force. After 3days, the again after 7 days, 7 rats in each group were sacrificed. Three days before they were sacrificed, Alizarin red S was intraperitoneally injected, and their maxillary bone, tibia and blood from their hearts were taken. The components of the extracted blood were biochemically analyzed and non-decalcified grinding resin sections for maxillary bone and tibia were made. The sections were examined with a polarization microscope, and fluorescent microscope. Smaller concentrations of Ca and P, the inorganic substances closely related to bone density, were found in the extracted blood of the OS group. Both OS and SS groups showed a possibility of bone remodeling with a high concentration of ALP after 7 days. An increase in bone density on the tension and compression sides of the microscrew and the tension side of the tooth for both OS and SS groups was confirmed with a polarization microscope. However, the bone density of the pressure side of the tooth and apical side was decreased. More deposits of Alizarin red S in the bone after 7 days rather than 3 days seen with a fluorescent microscope suggested the existence of new bone formation.

입체특이성 반응을 나타내는 키랄성 다섯자리 리간드, 1,9-bis(S)-prolyl-1,9-dioxo-2,5,8-triaza-nonane(S,S-prochen)를 S-proline과 diethylenetriamine (dien)으로부터 합성하여 $^{13}C-NMR$스펙트럼으로 확인하고, $CoCl_2{\cdot}6H_2O$와 반응시켜 적자색의 착물인 $[Co(SS-prodien)H_2O]ClO_4$ 를 합성하였다. 원소분석, 전자흡수스펙트럼, $^{13}C-NMR$스펙트럼 자료에 따라 S,S-prodian은 5자리로 배위되면서 중심의 dien부분은 fac형태로 결합하고, 한자리 리간드인 $H_2O$는 dien에 있는 2차질소원자에 대하여 cis 위치에 존재하는 ${\alpha}{\beta}$ (ffm)-형임을 알게되었다. 또한 CD스펙트럼에 따라 킬레이트된 리가드는 입체선택적으로 배위되어 ${\Lambda}$-${\alpha}{\beta}$ (ffm)-$[Co(SS-prodien)H_2O]^+$의 절대구조를 갖는다고 생각하였다.

A chiral pentadentate ligand, 1,9-bis(S)-prolyl-1,9-dioxo-2,5,8-triaza-nonane, (S,S-prochen) which shows the stereospecific reaction was synthesized from the reaction of S-proline and diethylenetriamine (dien). The red-violet $[Co(SS-prodien)H_2O]ClO_4$ was prepared by the oxidation of the aqueous solution dissolving $CoCl_2{\cdot}6H_2O$ and S,S-prodien. Elemental analysis, electronic absorption spectroscopy, and $^{13}C-NMR$ spectroscopy suggest that the geometrical structure of the Co(III) complex to be an ${\alpha}{\beta}$ (ffm) form, where the dien moiety of the ligand chelates the metal center to comprise a facial isomer, and an aqua ligand coordinates a cis site to the secondary nitrogens of the dien. Based upon the CD spectroscopic analysis, it seems that the absolute configuration of the ${\alpha}{\beta}$(ffm)-$[Co(SS-prodien)H_2O]ClO_4$ has the ${\Lambda}$-form.

국산 포도로부터 분리 된 Saccharomyces cerevisiae MM10, SS89 그리고 SS812 균주를 전체원료의 15%에서 배양하여 얻은 균체를 24 $^{\circ}Brix$로 동결농축한 사과액에 접종하여 무가당 사과주를 양조하였다. 발효 중 환원당은 대조균주와 비교 시 완만한 감소를 나타내었고, 총산은 모든 효모군에서 알코올 발효가 진행될수록 증가하였다. 최종 알코올 함량은 모든 효모군에서 12~13%의 함량을 나타내었다. 사과주의 유기산 함량의 범위는 2.43~2.92%이었으며, 유리당 조성은 약간의 과당이 검출된 SS89 균주를 제외한 모든 효모군에서 솔비톨만이 검출되었다. 메탄올은 검출되지 않거나 아주 극미량이 존재하였고, 알데히드는 107.68~114.27 ppm을 나타내었다. 퓨젤유에 있어서 propanol은 극미량 존재하였고, ethyl acetate는 55.27~69.54 ppm을 나타내었다. Isobutanol과 butanol은 각각 40.49~54.65 ppm과 25.47~27.73 ppm의 함량을 나타내었다. 그리고 isoamy1 alcohol은 108.88~217.26 ppm을 나타내었다. 총 페놀성 화합물 함량은 0.01~0.16%를 나타내었으며, hue 값은 1.40~2.01, intensity는 0.30~0.45의 범위를 나타내었다 명도는 91.78~98.19의 범위를 보였고, 적색도는 적색과 녹색의 중간색의 양상을 보였으며, 황색도는 2.38~7.7의 범위를 나타내었다. 관능검사 결과 색은 SS812 사과주, 향기는 SS889 사과주, 종합적인 기호도는 SS812 사과주가 가장 좋은 것으로 나타났다. 맛은 모든 선발균주가 동일하게 대조균주보다 높은 점수를 얻었다.

In this study, the good brewing conditions for the 24 $^{\circ}Brix$ freeze-concentrated apple wine were investigated. The four selected Saccharomyces cerevisiae strains MM10, SS89, SS812, and WW108, could ferment quickly when brewed with high sugar levels. During the fermentation, the reducing sugar contents slowly declined while the total acid in all the yeasts increased and the final alcohol content was 12-13%, a typical wine's alcohol content. The viable counts were shown to be 6-6.8 log cfu/ml. During the fermentation, the organic acid content was shown to be within the range of 2.36-3.11%, and the free sugar content, except for the SS89 and WW108 strains, was shown to consist only of sorbitol, although fructose was somewhat detected in the SS89 and WW108 strains. Methanol was not detected, or only a trace of it was detected, and the aldehyde content was 107.68-114.27 ppm. As for the fusel oil contents, a trace of propanol was detected. Isobutanol and butanol were present in 40.16-54.65 and 25.47-27.73 ppm, respectively. The isoamy1 alcohol content was shown to be the highest (108.88-217.26 ppm). The final total phenolic compounds were shown to be 0.1-0.16%. The final Hue values were shown to be 1.3-3.6, and the final intensity was 0.1-0.45. The lightness (L) was within the range of 91.78-98.51, the redness (a) was at a neutral position at red and green, and the yellowness (b) was within the range of 2.38-7.7. In the sensory evaluation, the SS812 strain was found to be the best in terms of color, the SS89 strain in terms of odor, and the WW108 strain in terms of taste. Overall, SS812 was found to be the best apple wine.

GOCI(정지궤도 해색센서) 해수환경분석 알고리즘들은 해양 광 특성 현장관측 자료들을 이용하여 개발되었다. 사용된 자료는 1998년부터 2009년까지 한반도 주변 해역에서 총 1348개 정점에서 얻어진 엽록소 농도(Chl-a), 부유물 농도(SS), 용존유기물의 흡광계수($a_{dom}$), 원격반사도($R_{rs}$) 현장자료들이다. GOCI 엽록소 농도 산출 알고리즘(GOCI Chl-a)은 부유물과 용존유기물의 영향을 모두 고려하고 네 개의 원격반사도 밴드비를 이용하여 개발하였다. GOCI Chl-a 알고리즘은 다른 알고리즘들보다 현장관측자료에 근사한 엽록소 농도 값을 산출하였다. SeaWiFS 영상자료에서 GOCI Chl-a 알고리즘은 SeaWiFS 표준 엽록소 산출 알고리즘들보다 평균 46 % 정도 보정된 엽록소 농도 값을 산출하였다. GOCI 부유물 농도 산출 알고리즘(GOCI SS)은 보편적인 두 개의 원격반사도 밴드비를 사용하지 않고, Ahn et al.(2001)의 원격반사도 단일밴드 방법을 사용하여 개발하였다. GOCI 용존유기물 산출 알고리즘(GOCI $a_{dom}$)은 원격반사도 밴드비 $R_{rs}(412)/R_{rs}(555)$와 $a_{dom}(\lambda)$)의 상관관계를 이용하여 개발하였다. GOCI 엽록소 형광 알고리즘과 GOCI 적조분석 알고리즘은 Ahn and Shanmugam(2007)와 Ahn and Shanmugam(2006)의 연구들에 의해 각각 개발되었다. 2010년 6월경에 GOCI의 성공적인 발사가 이루어지면 추후 GOCI 자료의 검보정 연구를 통해 개발된 알고리즘들의 문제점을 분석하고, 한반도 주변 해역의 해양 광 특성 현장자료의 지속적인 업데이트를 통한 알고리즘들의 개선작업이 이루어질 것이다.

Several ocean color algorithms have been developed for GOCI (Geostationary Ocean Color Imager) using in-situ bio-optical data sets. These data sets collected around the Korean Peninsula between 1998 and 2009 include chlorophyll-a concentration (Chl-a), suspended sediment concentration (SS), absorption coefficient of dissolved organic matter ($a_{dom}$), and remote sensing reflectance ($R_{rs}$) obtained from 1348 points. The GOCI Chl-a algorithm was developed using a 4-band remote sensing reflectance ratio that account for the influence of suspended sediment and dissolved organic matter. The GOCI Chl-a algorithm reproduced in-situ chlorophyll concentration better than the other algorithms. In the SeaWiFS images, this algorithm reduced an average error of 46 % in chlorophyll concentration retrieved by standard chlorophyll algorithms of SeaWiFS. For the GOCI SS algorithm, a single band was used (Ahn et al., 2001) instead of a band ratio that is commonly used in chlorophyll algorithms. The GOCI $a_{dom}$ algorithm was derived from the relationship between remote sensing reflectance band ratio ($R_{rs}(412)/R_{rs}(555)$) and $a_{dom}(\lambda)$). The GOCI Chl-a fluorescence and GOCI red tide algorithms were developed by Ahn and Shanmugam (2007) and Ahn and Shanmugam (2006), respectively. If the launch of GOCI in June 2010 is successful, then the developed algorithms will be analyzed in the GOCI CAL/VAL processes, and improved by incorporating more data sets of the ocean optical properties data that will be obtained from waters around the Korean Peninsula.

본 연구는 가축분뇨의 오염물질의 농도와 영상처리를 통해 얻어 낸 영상정보값과의 상관관계를 구하기 위해 수행하였다. CCD-카메라와 개인용 컴퓨터를 사용하여 축 분뇨의 영상을 받은 후 각 활성 영역에서의 영상정보와 공정시험 법으로 구한 오염물질과의 관계를 회귀분석법을 이용하여 다음과 같은 결론을 얻을 수 있었다. 1. 영상정보 R값은 CDD값과 결정계수가 0.9213으로 가장 높았으며, G값은 BOD값과 결정계수가 0.9019로 가장 높게 나타났으며, B값의 경우 오염물질 농도와의 상관관계는 매우 적은 것을 알 수 있었다. 2. H값은 BOD와의 결정계수($R^2$)가 0.9496으로 매우 높게 나타났다. 이는 BOD추정에 이용가능할 것으로 판단되었다. 3. BOD농도 추종을 위해서 G값,GRAY, H값, S값, 1값 등이 결정계수가 0.8이상을 나타냈으며, 그 중 H값이 가장 높은 결정계수를 나타냈으며, 이러한 정보를 이용하여 농도 추정이 가능할 것으로 판단되었다. 4. COD농도 추정을 위해서 R값, GRAY값, S값이 0.8이상의 결정계수를 나타냈으며, 그 중에서 R값과의 관계가 가장 높게 나타났으며, 이를 이용하여 간접적으로 농도 추정이 가능할 것으로 판단되었다. 5. SS농도와 영상정보값과 결정계수가 모두 0.8이하로 나타났으며, $NH_4$-N와 $NO_3$-N의 농도와 영상정보와의 결정계수는 모두 0.2이하로 매우 낮게 나타났으며, 가시광선영역의 CCD-카메라를 이용한 농도추정은 불가능할 것으로 판단되었다.

This study was conducted to find out the coefficient relationships between intensity values image processing and pollution density of slurries. Slurry images were obtained from the image processing system using personnel computer and CCD-camera. Software, written in Visual $c^{++}$, combined the functions of the image capture, image processing and image analysis. The data of image processing for slurries were analyzed by the method of regression analysis. The results are as follows. 1. Red(R)-values among image processing data were obtained the highest correlation coefficient 0.9213 for detecting COD. Also, green(G)-value were obtained the highest correlation coefficient 0.9019 fur detecting BOD. Blue(B)-value could not find significant values to detect the pollution resources density. 2. Hue(H)-values among image processing data were obtained the highest correlation coefficient 0.9466 for detecting BOD. This fact could be used in detecting BOD 3. Green(G)-value, GRAY-value, Hue(H)-value, Saturation(5)-value and Intensity(I)-value were the correlation coefficient more than 0.8 for BOD. Hue(H)-value was higher correlation coefficient than any other value. It was possible to detect pollution density of slurries by using the image processing system. 4. Red(R)-value, GRAY-value and Saturation(5)-value were obtained the correlation coefficient more than 0.8 for detecting COD. a-value had the highest correlation coefficient Among these values. It was possible to detect density indirectly by using the image processing system. 5. SS-density were obtained the correlation coefficient less than 0.8 by using the image processing system. The density of $NH_4$-N and $NO_3$-N were obtained correlation coefficient less than 0.2.

본 연구에서는 최근 해양, 대기, 육지환경을 동시에 관측할 수 있는 미국 첨단관측 위성 Terra/MODIS(moderate-resolution imaging spectroradiometer) 자료와 동중국해 북부해 역에의 현장 관측치를 이용하여 동중국해에서 탁한 해수의 수평 분포에 대한 동 하계 변동 규모를 정량화하고자 하였다. 국립수산과학원에서 2001년 6월부터 실시간 직접 수신한 MODIS 위성의 36개 채널 자료 중, 공간해상도가 250m인 채널 1(620~670nm) 및 공간해상도 500m인 채널 4(545~565nm)와 채널 3(459~479nm)을 합성한 RGB(red, green, blue) 디지털 영상자료를 이용하였다. 중국 연안측과 같이 수심이 얕은 동중국해역에서 발생되는 탁수의 수평 공간 분포 영역은 동계의 경우 강한 풍속과 수온약층의 소멸현상 등으로 수평면적이 평균 $232,000km^2$였다. 하계의 경우 비교적 약한 풍속과 강한 수온약층의 존재 등으로 수평면적이 평균 $71,000km^2$로 나타나, 동계 탁수의 수평적 분포 면적이 하계보다 약 3배 커지는 것으로 나타났다. 탁수의 수평면적 변동은 하계의 경우 10~20m 등수심선과, 동계의 경우 50m 등수심과 대략적으로 일치하는 분포를 보였다. 동계는 50m 이천까지의 수심에서 재부상한 부유물질이 표층에서 바람 또는 이류에 의해 50m 수심보다 깊은 해역의 표층으로 수평확산되어 탁수가 단지 표층에 국한되어 분포하는 것으로 추정되었다.

The spatiotemporal distribution of resuspended solid on the shelf of the southern Yellow Sea and the northern East China Sea was studied. The sea surface reflectance imageries obtained by remote sensing using satellite at channels of red (620~670nm), green(545~565nm) and blue(459~479nm) from Terra MODIS were used to explain the front of the high concentration suspended solid(SS) on the shelf in the East China Sea. The horizontal distribution of the resuspended solid was depended on the wind force, tidal current and stratification of water. The horizontal distribution areas of the resuspended solid in winter season during January~April, 2002 were three times wider than those in summer season during June~September, 2001.

무궁화의 종내(終內) 일대잡종(一代雜種)에서 화색(花色)과 화형(花型)의 분리현상(分離現象)을 조사(調査)할 목적(目的)으로 품종간(品種間) 교배(交配)를 하여 다음과 같은 결과(結果)를 얻었다. 1. 화판(花瓣)의 자주색(紫朱色)은 백색(白色)에 대(對)하여 dominance하고 세포질(細胞質) 유전(遺傳)에 의(依)한 것이라 추측(推測)된다. 2. 화판(花瓣)의 홑과 겹은 Ss, dd인자(因子)에 의(依)하여 발생(發生)한다. 3. 배수체(倍數？)무궁화와의 $F_1$의 화색(花色) 화형(花型)에는 큰 변화(變化)가 없었다. 4. Open-pollination되어 얻은 $F_1$에서 화색(花色)에 많은 변이(變異)가 있었다. 5. 유색(有色)견 화판(花瓣)무궁화와의 $F_1$에서는 화판(花瓣)에 적색(赤色) 반문(斑紋)이 있는 변이개체(變異個？)가 다수(多數) 발생(發生)되었다.

We observed performance of segregation in flower color and type of hybrids which obtained from crossing of intra species in Hibiscus syriacus. Obtained results were followings. 1. The purple flower was dominance to the white one and this was presumed that was owing to cytoplasmic heredity. 2. Single and double petal of flower was presumed that was originated from factors of Ss and dd. 3. There was not variation in flower color and type of $F_1$ hybrid between 4 n and 2 n Hibiscus syriacus. 4. There were many variation of flower color among $F_1$ hybrids which abtained in open pollination of Hibiscus syriacus. 5. We could observe many flowers variegated with red color among $F_1$ hybrids which obtained in crossing between double petal flowers of Hibiscus syriacus.

전 세계적으로 녹조 대발생은 빈번하게 보고되고 있으며, 국내에서도 매년 녹조로 인한 심각한 수질 오염 문제가 발생하고 있다. 지속적인 관리와 신속한 대응을 통한 수생태계 보호가 필요하다. 녹조 발생의 지표인 chlorophyll-a (Chl-a) 농도를 예측하기 위해 위성 영상을 이용한 연구가 많이 이루어지고 있다. 하지만 수계에 따라 변하는 분광특성과 대기 보정 오류로 인해 정확한 Chl-a 산출에 어려움이 있어 최근 머신러닝 모델을 활용하고 있다. 위성 분광지수 뿐만 아니라 녹조에 영향을 미치는 인자들에 대한 복합적인 고려가 필요하다. 따라서, 본 연구는 수질, 수문 및 기상 인자와 Sentinel-2 영상을 복합적으로 고려하여 데이터셋을 구축하였다. 최근 5년간 낙동강에 위치한 8개 보 구간의 Chl-a 농도 예측에 대표적인 앙상블 모델 random forest (RF)와 extreme gradient boosting (XGBoost)을 활용하였다. 모델 평가 지표로 r-squared score (R²), root mean square errors(RMSE), mean absolute errors (MAE)를 사용하였으며, XGBoost의 R²가 0.810, RMSE가 6.612, MAE가 4.457로 유의미한 결과를 얻은 것을 확인하였다. Shapley additive explanations (SHAP) 분석을 통해 두 모델 모두 수질 인자 suspended solids (SS), biochemical oxygen demand (BOD), dissolved oxygen (DO)과 red edge 밴드를 활용한 밴드비가 높은 중요도를 보인 것을 알 수 있었다. 다양한 입력 데이터는 모델 성능 향상에 도움을 주는 것을 확인할 수 있었으며, 국내외 녹조 탐지에 적용될 수 있을 것으로 보인다.

Algal bloom outbreaks are frequently reported around the world, and serious water pollution problems arise every year in Korea. It is necessary to protect the aquatic ecosystem through continuous management and rapid response. Many studies using satellite images are being conducted to estimate the concentration of chlorophyll-a (Chl-a), an indicator of algal bloom occurrence. However, machine learning models have recently been used because it is difficult to accurately calculate Chl-a due to the spectral characteristics and atmospheric correction errors that change depending on the water system. It is necessary to consider the factors affecting algal bloom as well as the satellite spectral index. Therefore, this study constructed a dataset by considering water quality, hydrological and meteorological factors, and sentinel-2 images in combination. Representative ensemble models random forest and extreme gradient boosting (XGBoost) were used to predict the concentration of Chl-a in eight weirs located on the Nakdong river over the past five years. R-squared score (R²), root mean square errors (RMSE), and mean absolute errors (MAE) were used as model evaluation indicators, and it was confirmed that R² of XGBoost was 0.80, RMSE was 6.612, and MAE was 4.457. Shapley additive expansion analysis showed that water quality factors, suspended solids, biochemical oxygen demand, dissolved oxygen, and the band ratio using red edge bands were of high importance in both models. Various input data were confirmed to help improve model performance, and it seems that it can be applied to domestic and international algal bloom detection.

실환경에서는 비래염분 뿐만 아니라 자외선, 산성비, 산업공해 부유 물질 등의 여러 가지 복합 열화요소에 노출되어 있어 강재가 발청(發錆)하는 임계 비래염분량을 찾기에 어려움이 있다. 본 연구에서는 비래염분 시뮬레이터를 사용하여 인위적으로 비래염분 부착량을 조절하였으며 강재 마감별 5종을 대상으로 부식촉진 시험을 실시하였다. 그 결과 NC, UC, RLC-1, SS201에 각각 0.58~0.73, 7.89~8.46, 57.95~69.48, $80.73{\sim}89.35mg/dm^2$의 비래염분량을 부착시켰을 때 발청하는 것을 확인할 수 있었으며, 발청 후 부식속도는 각각 1.60, 0.36, 0.97, 0.17로 NC가 가장 빠른 부식속도를 가지는 것으로 평가 되었다.

Many studies have investigated the airborne chlorides that can weaken the overall durability of the concrete structures due to the corrosion of steel materials, but most of the studies have aimed to examine weathering by exposing various construction materials to the actual oceanic environment. However, with the exposure test, it was difficult to find the threshold of precise corrosive amount of airborne chlorides due to diverse deteriorating environmental factors such as ultraviolet ray, acid rain, floating material from industrial pollution as well as airborne chlorides. Therefore, in this study, an airborne chloride simulator was set up, in oder to conduct a corrosion accelerating test for steels coated by five different finishing materials. As results, it was found that the corrosion began to be observed at $0.58{\sim}0.73mg/dm^2$ for no-coated steel, at $7.89{\sim}8.46mg/dm^2$for urethane-coated steel, at $57.95{\sim}69.48mg/dm^2$ for red lead-coated steel, and at $80.73{\sim}89.35mg/dm^2$ for stainless-coated steel, respectively. Hence, these specific data can be considered as the threshold ranges of corrosion for each coating material for steel.

본 연구에서 시험한 두 가지 농약에 대한 피부노출 측정 및 분석방법을 시험하였고 두 가지 농약에 대한 측정 및 분석방법으로써 모두 적정함을 확인하였다. 두 가지 농약에 대하여 호흡노출의 측정 및 분석법에 관한 타당도를 시험한 결과 본 연구방법으로 chlorpyrifos는 만족하였고 chlorothalonil의 경우 피부노출 측정 및 분석법만 타당하였다. 이와 같이 확정한 노출 측정방법으로 배 방제작업과 노지고추 방제작업시 농약노출량을 측정하였고 여러 가지 요인별로 비교하였나 두 가지 농약에 대하여 각 작목, 살포시기, 노출평가(피부,호흡기)와 작업용 변수로하여 노출평가를 실시하였다. 피부노출량은 과수 동력분무기, 노지고추 동력분무기 > 과수 SS기 > 동력분무기 보조의 순서로 나타났다. 과수 동력분무기와 노지고추 동력분무기 살포간 노출량의 차이는 유의하지 않았다. 과수나 노지고추의 방제만을 볼 때 다른 요인보다는 살포기기가 피부노출에 영향을 주는 주된 요인인 것으로 파악된다. 피부노출에서 작목은 주로 노출부위에 영향을 주는 것으로 평가되었다. 보조작업의 경우 손노출이 66%로 가장 높게 나타났다. 호흡기노출량은 각각의 노출이 서로 다르다고 할 수 없었다. 위험도 평가결과 chlorpyrifos 살포작업은 위험한 것으로 나타났으며 노출농도를 약 1/5 이하로 감소시킬 필요가 있는 것으로 밝혀졌다.

Objectives: The objectives of the present study were to establish the exposure monitoring method of chlorpyrifos and chlorothalonil application to pear and field red pepper by vehicle-mounted sprayer, hand-held sprayer and to assess the risk. Methods and Results: Chlorpyrifos met all of requirements of sampling and analysis method(U.S. EPA), but chlorothalonil met only dermal patch method. Potential dermal and inhalation exposure was evaluated in 42 farmers. Compared with the hand-held sprayer application, vehicle mounted sprayer demonstrated producing relatively lower dermal exposure levels with statistical significance. In dermal exposure during hand-held application, there is no significant difference between pear and red pepper. Conclusions: This results show that application method is the main factor of dermal exposure. There was no statistically significant difference between each group of respiratory exposure level. The margin of safety (MOS) was calculated to assess the risk of pesticide exposure. All Chlorpyrifos MOS values are lower than 0.2, which mean working conditions are unsafe. In order to protect farmers, big efforts to control exposure are needed.

1985년 7월에서 10월 사이에 부산연안지역에서 수집한 해수, 낙지, 피조개, 게, 우렁쉥이 등 70종의 시료에서 Vibrio vulnificus의 검출률과 시료에서 분리된 균의 세균학적 특성을 조사한 결과는 다음과 같다. 1. Vibrio vulnificus의 월별 검출률은 수온이 높은 8월에 $19.2\%$로 가장 높았으며 7월에 $7.7\%$ 10월에 $5.5\%$로 수온과 비례하여 나타났으며 7월과 10月 사이의 평균검출률은 $11.4\%$였다. 시료별 검출률은 서식해역의 오염도가 높고 오염된 해수와의 접촉부위가 많은 피조개와 게에서 $20\%$로 가장 높았으며 낙지 해수의 순으로 나타났다. 2. 시료에서 분리된 균과 환자에게 분리된 균의 생화학적인 차이점은 없었으며, 분리된 본균이 다른 Vibrio균과 구별될 수 있는 생화학적 특징은 ONPG 가수분해양성, lactose 분해양성, 식염내성이 $7\%$이하인 점이다. 3. 이균의 최적배양조건은 pH 8.0, 온도 $35^{\circ}C$부근이었으며, 배양온도별 비증식속도와 평균세대시간은 $35^{\circ}C$진탕배양에서 $1.21\;hr^{-1}$, 34min으로 가장 빨랐으며 $35^{\circ}C$ 정치배양, $40^{\circ}C$ 정치배양, $25^{\circ}C$ 진탕배양 $25^{\circ}C$ 정치배양의 순이었다. 4. 이 균은 장내세균분리용배지인 EMB, SS, DC 한천배지에서는 증식하지 못했으며 MC 한천배지상에서의 집낙의 특징은 적색 혹은 무색으로 나타났고 Endo 한천배지상에서는 적색, TCBS 한천배지상에서는 녹색집낙으로 나타났다. 5. 이 균을 $4^{\circ}C$에 냉장하였을 경우 매 16시간마다 약 1 log cycle씩 감소하였으며 $-18^{\circ}C$에 동결하였을 때는 거의 다 사멸하였다.

Vibrio vulnificus is a recently recognized halophilic organism that nay cause serious human infections. Patients infected with V. vulnificus often have a history of exposure to the sea, suggesting that the organism may be common inhabitant of marine environment. The purpose of this experiment is to investigate the distribution and bacteriological characteristics of V. vulnificus. The strain used in this experiment was isolated from sea water and sea products such as common octopus (Octopus variabilis), ark shell (Anadara broughtonii), blue crab (Ericheir japonica), and sea squirt (Synthia roretzi) collected in Pusan area from July to October in 1985. V. vulnificus was frequently isolated in August when temperature of sea water was around $26^{\circ}C$ and rarely isolated in October when temperature of sea water was around $18.5^{\circ}C$. The distinctive biochemical characteristics of V. vulnificus were ONPG hydrolysis positive and fermented lactose and not grown in peptone water contained $8\%$ NaCl. The optical density at 660 nm of the growth of V. vulnificus was reached maximum level after 8 hours of culture at $35^{\circ}C$ in brain heart infusion broth but that of V. vulnificus was little increased at $15^{\circ}C$ for 14 hours. Optimum temperature and pH for the growth of V. vulnificus were around $35^{\circ}C$ and 8.0. The specific growth rate and the generation time of V. vulnificus isolated from the samples were $1.21\;hr^{-1}$, 34 min at $35^{\circ}C$ and $0.61\;hr^{-1}$, 69 min at $25^{\circ}C$, respectively. V. vulnificus did not grow on eosin-methylene-blue agar, salmonella-shigella agar, deoxycholate agar but grew well on Endo agar, xylose-lysine-deoxycholate agar and hektoen enteric agar. On Endo agar, the colonies of V. vulnificus were red and achieved a diameter of 2 to 4 mm as a feature enabling differentiation of V. vulnificus from other Vibrio spp. V. vulnificus grow well on TCBS agar forming green colonies. V. vulnificus refrigerated at $4^{\circ}C$ exhibited a linear decline of its viablity as 1 log cycle in every 16 hours storage, while V. vulnificus freezed at $-18^{\circ}C$ almost became extinct.

1985년 7월에서 10월 사이에 부산연안지역에서 수집한 해수, 낙지, 피조개, 게, 우렁쉥이 등 70종의 시료에서 Vibrio vulnificus의 검출률과 시료에서 분리된 균의 세균학적 특성을 조사한 결과는 다음과 같다. 1. Vibrio vulnificus의 월별 검출률은 수온이 높은 8월에 $19.2\%$로 가장 높았으며 7월에 $7.7\%$ 10월에 $5.5\%$로 수온과 비례하여 나타났으며 7월과 10月 사이의 평균검출률은 $11.4\%$였다. 시료별 검출률은 서식해역의 오염도가 높고 오염된 해수와의 접촉부위가 많은 피조개와 게에서 $20\%$로 가장 높았으며 낙지 해수의 순으로 나타났다. 2. 시료에서 분리된 균과 환자에게 분리된 균의 생화학적인 차이점은 없었으며, 분리된 본균이 다른 Vibrio균과 구별될 수 있는 생화학적 특징은 ONPG 가수분해양성, lactose 분해양성, 식염내성이 $7\%$이하인 점이다. 3. 이균의 최적배양조건은 pH 8.0, 온도 $35^{\circ}C$부근이었으며, 배양온도별 비증식속도와 평균세대시간은 $35^{\circ}C$진탕배양에서 $1.21\;hr^{-1}$, 34min으로 가장 빨랐으며 $35^{\circ}C$ 정치배양, $40^{\circ}C$ 정치배양, $25^{\circ}C$ 진탕배양 $25^{\circ}C$ 정치배양의 순이었다. 4. 이 균은 장내세균분리용배지인 EMB, SS, DC 한천배지에서는 증식하지 못했으며 MC 한천배지상에서의 집낙의 특징은 적색 혹은 무색으로 나타났고 Endo 한천배지상에서는 적색, TCBS 한천배지상에서는 녹색집낙으로 나타났다. 5. 이 균을 $4^{\circ}C$에 냉장하였을 경우 매 16시간마다 약 1 log cycle씩 감소하였으며 $-18^{\circ}C$에 동결하였을 때는 거의 다 사멸하였다.

Vibrio vulnificus is a recently recognized halophilic organism that nay cause serious human infections. Patients infected with V. vulnificus often have a history of exposure to the sea, suggesting that the organism may be common inhabitant of marine environment. The purpose of this experiment is to investigate the distribution and bacteriological characteristics of V. vulnificus. The strain used in this experiment was isolated from sea water and sea products such as common octopus (Octopus variabilis), ark shell (Anadara broughtonii), blue crab (Ericheir japonica), and sea squirt (Synthia roretzi) collected in Pusan area from July to October in 1985. V. vulnificus was frequently isolated in August when temperature of sea water was around $26^{\circ}C$ and rarely isolated in October when temperature of sea water was around $18.5^{\circ}C$. The distinctive biochemical characteristics of V. vulnificus were ONPG hydrolysis positive and fermented lactose and not grown in peptone water contained $8\%$ NaCl. The optical density at 660 nm of the growth of V. vulnificus was reached maximum level after 8 hours of culture at $35^{\circ}C$ in brain heart infusion broth but that of V. vulnificus was little increased at $15^{\circ}C$ for 14 hours. Optimum temperature and pH for the growth of V. vulnificus were around $35^{\circ}C$ and 8.0. The specific growth rate and the generation time of V. vulnificus isolated from the samples were $1.21\;hr^{-1}$, 34 min at $35^{\circ}C$ and $0.61\;hr^{-1}$, 69 min at $25^{\circ}C$, respectively. V. vulnificus did not grow on eosin-methylene-blue agar, salmonella-shigella agar, deoxycholate agar but grew well on Endo agar, xylose-lysine-deoxycholate agar and hektoen enteric agar. On Endo agar, the colonies of V. vulnificus were red and achieved a diameter of 2 to 4 mm as a feature enabling differentiation of V. vulnificus from other Vibrio spp. V. vulnificus grow well on TCBS agar forming green colonies. V. vulnificus refrigerated at $4^{\circ}C$ exhibited a linear decline of its viablity as 1 log cycle in every 16 hours storage, while V. vulnificus freezed at $-18^{\circ}C$ almost became extinct.

본 연구는 산림생태복원 사후모니터링 대상지 150개소를 대상으로 산림생물다양성 유지·증진, 산림생태계의 보전·관리와 산림복원용 자생식물의 실효성을 제고해볼 수 있는 기초자료로 활용하기 위하여 조사를 수행하였다. 조사는 2020년 4월부터 2023년 10월까지 계절별로 각 1~2회씩 수행하였다. 한국의 산림생태복원 사후모니터링 대상지 150개소의 관속식물상은 139과 599속 1,171종 27아종 92변종 11품종 11잡종 5재배품종 총 1,317분류군으로 확인되었다. 150개소 전체 조사 면적은 약 5.7km²로 우리나라 전체 산림면적 62,860km²의 0.009%에 불과하지만, 우리나라 관속식물의 4,724종 중에서 27.9%가 출현한 것으로 나타났다. 특기할만한 식물은 멸종위기야생생물은 끈끈이귀개 1분류군, 희귀식물과 적색목록은 조도만두나무, 홍도까치수염 등 47분류군, 한반도 특산식물은 병꽃나무 등 55분류군, 식물구계학적 특정식물은 갈퀴아재비 등 총 324분류군이 조사되었다. 외래식물은 나도닭의덩굴, 털여뀌 등 123분류군(불확실종 2분류군 포함) 등이 확인되었고, 광분포종(WS)은 돌소리쟁이 등 48분류군, 심각한 확산종(SS)은 전동싸리 등 17분류군, 우려되는 확산종(CS)은 벳지 등 16분류군, 경미한 확산종(MS)은 큰석류풀 등 23분류군, 잠재적 확산종(PS)은 털큰참새귀리 등 17분류군으로 파악되었다. 산림복원지의 평균 귀화율은 9.3%와 도시화지수는 29.3%로 산출되었다. 산림복원지 사후모니터링 대상지에서 산림복원용 자생식물은 분비나무, 너도밤나무, 병아리꽃나무, 갯보리, 통보리사초 5분류군을 제외한 89분류군이 확인되었다. 산림복원은 자연적·인위적으로 훼손된 산림의 생태계 및 생물다양성이 원래의 상태에 가깝게 유지·증진될 수 있도록 그 구조와 기능을 회복시키는 것으로 산림생태복원 모니터링 체계수립을 위해 1단계(1년, 2년) 및 2단계(5년, 10년)뿐만 아니라 영구적인 모니터링이 수행되어야 할 것으로 판단된다.

This study carried out a survey targeting 150 post-monitoring sites for forest ecological restoration in order to use them as basic data to maintain and improve forest biodiversity and the effectiveness of native plants for forest restoration. The survey was conducted once or twice per season from April 2020 to October 2023. The vascular flora of 150 post-monitoring sites for forest ecological restoration in Korea was identified as 1,317 taxa in 139 families, 599 genera, 1,171 species, 27 subsp., 92 var., 11 f., 11 hybrids, and 5 cultivar. The total survey area of 150 locations is about 5.7 km², which is only 0.009% of Korea’s total forest area of 62,860 km², but 27.9% of the 4,724 taxa of vascular plants in the Korean Peninsula were found to have appeared. Remarkable plants include, Drosera peltata Thunb. var. nipponica (Masam.) Ohwi, 1 taxon for endangered wild species, 47 taxa for rare plants and red list plants, 55 taxa for plants endemic to the Korean Peninsula, and a total of 324 taxa for floristic target plants. 123 taxa of exotic plants were identified, and the average naturalization rate and urbanization index of the forest restoration area was calculated to be 9.3% and 29.3%. In the forest restoration post-monitoring site, 89 taxa of native plants for forest restoration were identified.

This study was conducted to investigate the nutritional characteristics of the following Korean-distributed fish species commonly consumed as sliced raw fish (CC-SRF): olive flounder Paralichthys olivaceus (OF), red sea bream Pagrus major (RS), Atlantic salmon (AS), coho salmon (CS) and sockeye salmon (SS). The crude protein and lipid contents of OF, RS, AS, CS and SS were 20.2% and 5.5%, 21.2% and 6.8%, 17.7% and 18.5%, 18.3% and 16.1%, and 20.4% and 5.7%, respectively. Regardless of the type and weight of fish species, the major amino acids were leucine, lysine, aspartic acid, and glutamic acid, whereas histidine was identified as a limiting amino acid. The major minerals in all CC-SRF were P, K and Se based on the recommended daily intake or sufficient intake for Korean males aged between 19-49 years. Among the different types of all CC-SRF, the intake of OF and SS lipids is predicted to be associated with a lower n-6/n-3 fatty acid ratio, whereas among the free amino acids, which are expected to have health functionality, we identified taurine in OF and RS, and anserine in salmons. The digestibility of OF, RS, AS, CS and SS were 60.7%, 54.9%, 48.5%, 49.6%, and 53.4%, respectively.

Sang-Bin Lee (2022). On the Translation of Form in Poetry: An Investigation of Kim Hyesoon’s Han chan ?i pulg?n k?ul (A Drink of Red Mirror). In this article, I discuss the matter of form in poetry translation. My argument is that the visual elements of a poem are not always translated as they appear in the source text and may instead be reconstructed by the translator’s interpretation of the poem. To clarify this argument, I compare Han chan ?i pulg?n k?ul, a collection of surrealist poems written by Kim Hyesoon, with A Drink of Red Mirror, an English translation by Jiwon Shin, Lauren Albin, and Sue Hyon Bae. Specifically, I examine seven poems ? Old Refrigerator (Oraedoen naengjanggo), Lunar Month’s End (K?m?m), O (O), Face (?lgul), Scribbled Letter (Kalgy? ss?n p’y?nji), Eye of a Typhoon (T’aep’ung ?i nun), and Mixer & Juicer (Mixer & Juicer) ? in terms of layouting, lowercasing, italicizing, or spacing. Then, I briefly touch on the “same” poems in Un verre de miroir rouge, a French translation by Choon-woo Yee and Lucie Angheben. This comparison demonstrates the stark differences in visuals between the English and French translations. (Hankuk University of Foreign Studies, South Korea)