Earticle

Objectives: To validate a critical-size mandibular bone defect model in miniature pigs. Materials and Methods: Bilateral notch defects were produced in the mandible of dentally mature miniature pigs. The right mandibular defect remained untreated while the left defect received an autograft. Bone healing was evaluated by computed tomography (CT) at 4 and 16 weeks, and by micro-CT and non-decalcified histology at 16 weeks. Results: In both the untreated and autograft treated groups, mineralized tissue volume was reduced significantly at 4 weeks post-surgery, but was comparable to the pre-surgery levels after 16 weeks. After 16 weeks, CT analysis indicated that significantly greater bone was regenerated in the autograft treated defect than in the untreated defect (P=0.013). Regardless of the treatment, the cortical bone was superior to the defect remodeled over 16 weeks to compensate for the notch defect. Conclusion: The presence of considerable bone healing in both treated and untreated groups suggests that this model is inadequate as a critical-size defect. Despite healing and adaptation, the original bone geometry and quality of the pre-injured mandible was not obtained. On the other hand, this model is justified for evaluating accelerated healing and mitigating the bone remodeling response, which are both important considerations for dental implant restorations.

Objectives: To validate a critical-size mandibular bone defect model in miniature pigs.Materials and Methods: Bilateral notch defects were produced in the mandible of dentally mature miniature pigs. The right mandibular defect remained untreated while the left defect received an autograft. Bone healing was evaluated by computed tomography (CT) at 4 and 16 weeks, and by micro-CT and non-decalcified histology at 16 weeks.Results: In both the untreated and autograft treated groups, mineralized tissue volume was reduced significantly at 4 weeks post-surgery, but was comparable to the pre-surgery levels after 16 weeks. After 16 weeks, CT analysis indicated that significantly greater bone was regenerated in the auto-graft treated defect than in the untreated defect (P=0.013). Regardless of the treatment, the cortical bone was superior to the defect remodeled over 16 weeks to compensate for the notch defect.Conclusion: The presence of considerable bone healing in both treated and untreated groups suggests that this model is inadequate as a critical-size defect. Despite healing and adaptation, the original bone geometry and quality of the pre-injured mandible was not obtained. On the other hand, this model is justified for evaluating accelerated healing and mitigating the bone remodeling response, which are both important considerations for dental implant restorations.

The porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1, and DQA) genes were used for investigation of SLA haplotypes in Yucatan miniature pigs in Korea. This pig breed is a well-known model organism for biomedical research worldwide. The current study indicated that Korean Yucatan pig population had 3 Class I haplotypes (Lr-4.0, Lr-6.0, and Lr-25.0) and 3 class II haplotypes (Lr-0.5, Lr-0.7, and Lr-0.25). The combinations of SLA class I and II haplotype together, 2 homozygous (Lr-4.5/4.5 and Lr-6.7/6.7) and 3 heterozygous (Lr-4.5/6.7, Lr-4.5/25.25, and Lr-6.7/25.25) haplotypes were identified, including previously unidentified new heterozygous haplotypes (Lr-4.5/4.7). In addition, a new SLA allele typing method using Agilent 2100 bioanalyzer was developed that permitted more rapid identification of SLA haplotypes. These results will facilitate the breeding of SLA homozygous Yucatan pigs and will expedite the possible use of these pigs for the biomedical research, especially xenotransplantation research.

급성신손상은 높은 이환율과 치사율을 나타내는 심각한 질환이며, 허혈-재관류에 의한 신손상은 급성신손상의 중요한 원인이 된다. 본 연구는 미니돼지에서 급성신손상을 진단하는데 임상적으로 유용한 바이오마커를 찾아내기 위해 실시되었다. 세 마리의 미니돼지에서 60분간 신동맥을 결찰하여 양측성 신허혈을 유도하였다. 각 미니돼지에서 결찰 전, 결찰 후 0, 1, 3, 5일에 혈액 및 뇨 검체를 채취하였다. 혈청 및 뇨 검체에서 BUN, creatinine, 나트륨 및 요산을 측정한 후 나트륨 및 요산의 분획배설율($FE_{Na}$, $FE_{UA}$)을 산출하였다. 또한 IL-6, IL-18, L-FABA 및 GST를 Western immunoblotting을 실시하여 측정하였다. 결과에서 세 마리 미니돼지 모두 혈청 BUN과 creatinine 농도는 결찰 후 1일째에 유의적으로 증가하였다. 그러나 $FE_{Na}$와 $FE_{UA}$는 현저한 개체차를 보였다. 수술 전과 후를 비교했을 때 허혈 이후의 뇨 검체에서는 IL-6, IL-18, L-FABP 및 GST의 농도가 유의적으로 증가하였다. 결론적으로, $FE_{Na}$와 $FE_{UA}$에 대해서는 추가적인 연구가 필요하다고 생각되며, 혈청 BUN, creatinine과 뇨 IL-6, IL-18, L-FABP 및 GST는 돼지의 허혈-재관류 모델에서 다른 바이오마커와 함께 급성신손상을 진단하는 민감한 바이오마커가 될 수 있을 것이라 생각된다.

Acute kidney injury (AKI) is a serious problem associated with high morbidity and mortality. Ischemia-reperfusion is an important cause of acute kidney injury. This study was performed to ascertain clinically useful biomarkers for the diagnosis of AKI. In three miniature pigs, AKI were induced by 60 minutes of bilateral renal ischemia by the clamping renal artery. Blood and urine samples were collected from the pigs prior to clamping (baseline) and 0, 1, 3 and 5 days post-clamping. Serum blood urea nitrogen (BUN), creatinine, sodium and uric acid were measured in serum and urine samples. Fractional excretion of sodium ($FE_{Na}$) and fractional excretion of uric acid ($FE_{UA}$) were calculated. Also, interleukin (IL)-6, IL-18, liver type fatty acid binding protein (L-FABP) and glutathione-S-transferase (GST) were detected by Western immunoblotting. Serum BUN and creatinine levels were increased significantly at day 1 post-clamping in all three miniature pigs. However, $FE_{Na}$ and $FE_{UA}$ showed marked individual differences. Western immunoblotting revealed significantly increased levels of IL-6, IL-18, L-FABP and GST in post-ischemic urine, compared to pre-clamping. While more research concerning the variance of $FE_{Na}$ and $FE_{UA}$ is needed, serum BUN, creatinine, IL-6, IL-18, L-FABP and GST may be sensitive urine biomarkers for diagnosis of AKI together with other biomarkers in the porcine ischemia-reperfusion model.

To accurately analyze the accidents in nuclear reactors, a thermohydraulic-neutronic coupling calculation is required to solve fluid dynamics and nuclear reactor kinetics equations in fine cells simultaneously and evaluate the local effects of neutronic and thermohydraulic parameters on each other. In the present study, a 3D thermohydraulic-neutronic coupling model is developed, validated and then applied for Isfahan MNSR (Miniature Neutron Source reactor) safety analysis. The proposed model is developed using FLUENT software and user defined functions (UDF) are applied to simulate the neutronic behavior of MNSR. The validation of the proposed model is first evaluated using 1mk reactivity insertion experiment into Isfahan MNSR core. Then, the developed coupling code is applied for a design basis accident (DBA) scenario analysis with the insertion of maximum allowed cold core reactivity of 4 mk. The results show that the proposed model is able to predict the behavior of the reactor core under normal and accident conditions with a good accuracy.

지구환경의 변화로 국내의 강우패턴이 변화하고 사회의 발달로 인한 여가시간을 증가로 산지 및 산지인근 지역에 대한 활용도가 높아짐에 따라 산지재해에 대한 관심도가 증가하고 있다. 산지재해 중 최근 이슈가 되고 있는 토석류재해는 산지에서 발생하여 피해가 하류지역까지 영향을 미치는 재해다. 토석류재해를 저감하기위한 목적으로 다양한 방법들이 동원되고 있지만 사방댐의 건설이 가장 큰 비중을 차지하고 있다. 본 연구에서는 산지환경 및 산지계류의 생태계에 영향을 미치는 사방댐의 건설에서 벗어나 환경 친화적이고 발생원에서의 재해위험을 저감할 수 있는 수목을 이용한 토석류 재해저감 효과를 확인하기 위해 수목 뿌리 모사모형을 제작하였다. 또한 수목 뿌리모사모형을 이용한 토석류 유출량 변화실험을 수행하여 수목 뿌리의 형태에 따른 저감효과를 분석하였다. 연구결과 다양한 수목뿌리 형태에 따른 토석류 저감효과를 제시하였다.

본 논문은 국내 주요 시설 및 국가 기반 시설로 변화되고 있는 터널 및 지하 공동구의 조기화재탐지설비시스템 설치에 이용되고 있는 광센서 선형 화재 감지 시스템에 대한 특성 분석을 목적으로 연구를 수행하였다. 연구 방법으로는 저온 환경인 $-20^{\circ}C$에서 5일간 5가지의 열감지기 중에서 작동 여부를 확인한 결과 광센서 선형 감지기만 작동하는 것을 알 수 있었다. 또한 터널과 같은 풍속이 발생되는 곳을 가정하여 축소 모형 터널을 제작하여 0 m/s와 1 m/s를 변수로 하여 광센서 선형 감지기의 작동 특성을 분석하였다. 그 결과로서 광센서 선형 감지 시스템의 온도 변화에 있어서는 풍속이 없을 때인 0 m/s 때의 값이 높게 나타나는 것을 알 수 있었다.

In this study, we conducted a low temperature operating test and miniature tunnel model test to study the fire detection capability and properties of an early fire detection system using an optical sensor linear detector that can be installed in harsh environments such as tunnel or utility-pipe conduits which are becoming the major and national infrastructure facilities. The test showed that the optical sensor linear detector was the only one functioned properly among five thermal detectors installed at a low temperature of $-20^{\circ}C$ for 5 days. To study were analyzed adaptability of optical sensor linear detector in the windy tunnel, the operating properties of the optical sensor linear detector when the wind velocity was varied between 0 m/s and 1 m/s in a miniature tunnel model. The temperature change was high when the wind velocity was 0 m/s.