Earticle

The objective of this work was to analyze the concentrations of progesterone (P4) and estrogen (E2) hormones changed during estrus synchronization in dairy heifers. Estrus synchronization was carried out with CIDRⓇ (Controlled Intravaginal Drug Release) devices. Corpus luteum (CL) was classified into three grades based on its size and palpable characteristics. The concentrations of P4 and E2 were measured by enzyme-amplified chemiluminescence. Serum P4 concentration was markedly low at the estrus stage (36 hrs after removal of CIDR) compared to other stages, while E2 concentration was kept high during estrus stage. The serum P4 concentration was highest in the CL classified into gradeⅠ. These results indicate that P4 concentration could be used as a criteria for determining recipients for artificial insemination or embryo transfer in dairy cattle.

본 연구는 한우 체세포를 이용하여 생산된 복제란을 한우 대리모에 이식하여 임신이 확인된 개체에서 임신 기간 중 주요 호르몬의 발현 특성을 인공수정으로 임신된 대리모와 비교 분석하고자 실시하였다. 한우 섬유아세포를 이용하여 생산된 체세포 복제란을 자연발정으로 동기화된 한우 대리모에 이식하여 임신이 확인된 개체를 공시하였으며(n=8), 대조군으로는 인공수정으로 임신된 대리모을 사용하였다(n=5). 발정 관찰 후 60일경에 직장검사로 임신을 확인하였다. 주요 스테로이드 호르몬인 progesterone(P4)와 estradiol-l7 (E2) 농도는 방사선동위원소 면역분석시험(RIA) 방법을 이용하였으며, 혈중 cortisol 농도는 ELISA 방법으로 측정하였다. 인공수정한 대리모의 경우 E2 농도가 분만 시기에 급격하게 증가하였으나, P4 농도는 분만 시기에 급격하게 감소하는 경향을 나타내었다. 이에 반해 복제란 이식우의 혈장 P4 농도는 분만 50일 전부터 분만 10일전까지는 대조군과 유사하게 유지되었으나, 분만예정일에는 전혀 떨어지지 않고 높은 수준으로 유지되었다. 한편, 복제란 이식우에서 분만 때까지 정상적으로 임신이 유지된 대리모들의 경우는 임신 기간 동안 cortisol 농도는 임신 후반기까지 낮게 유지되며 별다른 변화를 나타내지 않았다. 반면에 유산이 일어난 개체의 경우에는 임신 100일경에 cortisol의 농도가 급격하게 증가하는 것을 확인하였다. 이상의 결과를 종합하여 보면, 복제란 이식우의 경우 분만예정일 전 후에 일어나는 급격한 호르몬의 변화가 일어나지 않음을 확인할 수 있었으며, 이러한 현상은 복제란 이식우의 분만 지연과 밀접한 관계가 있는 것으로 사료된다.

This study was performed to analyze the characterization of plasma hormonal levels during pregnancy in the Hanwoo recipients pregnant by artificial insemination (AI) or somatic cell nuclear transfer (SCNT) embryos. The synchronized recipients pregnant by SCNT embryos produced by Hanwoo fetal fibroblast cells (n=8) and by AI (control, n=5) were used. The plasma hormonal levels were measured by RIA (P4 and E2) and ELISA (cortisol), respectively. In control, the increase of E2 and the decrease of P4 were occurred immediately before the initiation of parturition. The expression pattern of plasma P4 was similar in both groups from 50 to 10 days before parturition, however, it did not decrease even at the expected date of labor in the SCNT recipients. The plasma cortisol was expressed a lower level during pregnancy in the SCNT recipients. But, the cortisol was increased in the cow aborted around 100 days of pregnancy (n=1). Based on these results, it can be postulated that the failure of the hormonal changes immediately before parturition in the SCNT recipients may be one of the most important reasons for a delayed parturition in clone calving.

To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the effects on the water extracts of Artemisia princeps Pampanini (APP) on lipopolysaccharide-induced nitric oxide (NO), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) production by RAW 264.7 macrophage cell line. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased PGE2 and TNF-α production. Consistent with these results, the protein and mRNA expression level of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by water extracts of APP in a dose-dependent manner. These results suggest that APP may exert anti-inflammatory and analgesic effects possibly by suppressing the inducible NO synthase and COX-2 expressions.

Prostaglandin (PG) E2 is an important mediator of skin wound healing without excessive scarring and gastriculcer healing. However, PGE2 has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandindehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular PGE2levels in HaCaT cells and inhibited 15-PGDH (ED50 : 168.4 ㎍/mL) with relatively low cytotoxicity (IC50 : 250.0 ㎍/mL). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased andprostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE(168.4 ㎍/mL) was comparable to that of TGF-β1 (300 pg/mL) as a positive control. These results demonstrate that EFEEmay be valuable therapeutic materials for the treatment of PGE2 level dependent diseases.

코로나19 펜데믹이 장기화되면서 비대면 업무가 늘어나고 단순반복적인 질문 및 업무에 대한 자동화 수요가 증가하였으며 성과도 보고 있다. 따라서 기업은 더 나아가 E2E 업무에 복잡한 여러 업무 프로세스를 연계하고 위해 AI 등 다양한 기술을 적용하여 모든 작업을 자동화하려고 시도하고 자동화 업무영역을 확장하고 있다. 하지만 Intelligent Process Automation(IPA)부분으로의 확장은 아직 적용사례 및 관련 솔루션을 찾아보기 어려울 정도로 시작 단계에 머물고 있어 복잡하고 다양한 업무 프로세스들로 구성되어 있는 전사업무의 자동화를 고려하고 있는 기업에서는 도입에 대한 의사결정을 내리기에는 부족한 면이 있다. 본 연구에서는 이를 해결하기 위하여 RPA, Chatbot, AI 기술을 적용한 Hyperautomation Platform(HAP) 구성을 제안하였고, HAP를 사용하여 지능형 프로세스 자동화를 가져올 수 있는 적용방안과 현업 업무에 적용 가능한 구현사례를 제시하여 객관적이고 종합적으로 도입을 검토할 수 있도록 하였으며, Hyperautomation 개념의 실현 가능성을 확인하여 HAP를 적극적으로 활용할 수 있도록 하는데 의미와 가치가 있다.

As the COVID-19 pandemic is prolonged, non-contact work has increased, as well as the demand for automation of simple and repetitive questions and tasks with success of using them. Therefore, companies are attempting to expand the area of automated business and apply various technologies such as AI to complex and various business processes of E2E to provide automation of all business. However, the extension to Intelligent Process Automation (IPA) is still in its beginning stage so that it is difficult to find practical use cases and related solutions. In this aspect, it is safe to say that there is insufficient evidence for companies which have various and complex enterprise processes to make a decision about the adoption. In this study, to solve this problem, a Hyper Automation Platform (HAP) that consists of RPA, Chatbot, and AI technology was proposed. Moreover, an implementation method that can bring intelligent process automation using HAP, and practical use-cases were provided so that it makes it possible to review the implementation of the HAP objectively and comprehensively. This study is meaningful and valuable to check the feasibility of the Hyper Automation concept and to actively utilize HAP.

Although current RFID technologies can provide wide advantages in food industry area, most food companies do not consider willingly adopting this technology yet. This paper will present how the effective implementation of RFID systems in food industry could be realized. To Measure the importance of RFID systems, this study utilized the BMT(Bench Mark Test) methodology using RFID systems that were composed of tag and reader. After conducting this study, it suggests the effective tag attachment methods on the objects. The results are shown that the excellent tag product is a RFCamp and a fixed reader of Thingmagic Mercury 4 is the best product. Also, a portable reader of Motorola MC9090-RFID is recommend to the highest product. Therefore, this test is useful for selecting the best RFID systems to the food industry area. In addition, this study displays the effective tag attachment method to each bottle that was used to the experiments in food industry. Therefore, this study is contributed to the not only importance in the government IT areas as a growth engine but also the importance for consumer as a right for the knowledge about the food quality.

This study analyzes the feasibility of operating the E190-E2 aircraft at Ulleung Airport, which has a runway length of 1,200 meters. Using aircraft manufacturer data and PACE LAB under EASA standards, takeoff and landing performance were evaluated under various environmental conditions. Results indicate that with round-trip fuel tankering, the aircraft can carry up to 106 passengers at departure and 89 at arrival under wet runway conditions. If refueling is available at Ulleung Airport, the payload capacity can increase by approximately 10 passengers. A flight test conducted at Pohang Airport supported these findings. The study suggests that minor infrastructure improvements, such as refueling facilities and limited runway extension, would make E190-E2 operations at Ulleung Airport technically feasible.

비대면 교육 서비스의 효과를 높이기 위해서는 표준화된 웹 환경 제공, 정보보안 서비스 강화, 온라인 환경 가이드 개선, 교육 서비스 홍보, 신청, 프로그램 이용, 성과 확인까지 이용자와 상호작용 할 수 있는 플랫폼 확보 등과 같은 문제를 해결해야 한다. 재택근무나 원격교육 등 현실적으로 비대면 서비스의 핵심 적인 기술은 바로 화상회의라 할 수 있다. 화상회의는 비디오와 오디오 스트리밍 기술, 멀티미디어 생성 및 제어 기술, 효율적인 전송을 위한 압축기술, 이를 P2P(Peer-to-Peer) 혹은 중계 서버를 통해 실시간으 로 전달하는 전송기술 등 다양한 요소들이 포함되어 있다. 본 논문은 웹 기반 화상회의 시스템에서 종단 간 암호화 서비스 (E2EE: End-to-end Encryption)를 제공하기 위한 서비스 구조를 제안하였다.

In order to increase the effectiveness of non-face-to-face education services, problems such as providing a standardized web environment, strengthening information security services, improving online environment guides, and securing a platform for interaction with users must be solved. The core technology of non-face-to-face services such as telecommuting or distance education is video conferencing. Video conferencing includes various elements such as video and audio streaming technology, multimedia creation and control technology, compression technology for efficient transmission, and transmission technology that delivers it in real time through P2P (Peer-to-Peer) or a relay server. In this paper, a service structure is proposed to provide an end-to-end encryption service in a web-based video conferencing system.

This study empirically examines the spatial impact of the National Fire Hospital, currently under construction to address the healthcare infrastructure deficit in the central region of Chungcheongbuk-do. The research focuses on analyzing the transformation of regional accessibility and spatial equity within the healthcare delivery system. Methodologically, a dual spatial analysis framework is employed: a GIS-based OD Matrix analysis to measure physical connectivity via road networks, and the Enhanced 2-Step Floating Catchment Area (E2SFCA) model to assess potential spatial accessibility by accounting for the supply-demand interaction between hospital capacity and population distribution. Additionally, the Gini Coefficient is utilized to quantify the reduction of spatial disparities in healthcare resource distribution. The analysis demonstrates that the strategic placement of the National Fire Hospital significantly improves physical accessibility, effectively integrating Chungbuk Innovation City (Maengdongmyeon, Eumseong-gun; Deoksan-eup, Jincheon-gun) and its surrounding settlements into the hospital’s service catchment area. Furthermore, substantial spatial spillover effects were observed, expanding the accessible service area to include neighboring Jeungpyeong-gun and parts of Goesan-gun. Significantly, the reduction in the province-wide Gini Coefficient confirms that the new facility functions as a regional anchor, correcting the structural imbalance of medical resources. These findings underscore that the spatial allocation of public infrastructure is a critical determinant in enhancing settlement conditions and fostering balanced regional development.

Baengnyeong Airport is under review for construction to improve transportation accessibility in island regions and has passed the preliminary feasibility study. While airport development significantly enhances transportation convenience for residents, it may also cause aircraft noise issues and lead to conflicts within local communities. Previous studies estimated noise impacts based on ATR-42 and Q300 aircraft. However, this study focuses on a more realistic assessment using ATR-72 and E190-E2 aircraft. By utilizing the FAA’s Aviation Environmental Design Tool (AEDT), a projected noise contour map for Baengnyeong Airport was developed. The analysis shows that considering ATR-72 and E190-E2, which generate higher noise levels, provides a more practical evaluation of noise-affected areas. The results indicate that the noise impact is mostly confined within the runway area; however, potential noise complaints may arise from Baengnyeong Island and nearby regions. Based on these findings, this study suggests the need for optimized flight procedures and urban planning measures to mitigate aircraft noise issues.

본 예비연구의 목적은 이미지 생성 인공지능 도구인 DALL-E2를 활용하여 영어 작문 활동을 실시한 후 중학 생들의 영어 학습에 미치는 반응을 살펴보는 데 있다. 이를 위하여 15명의 중학교 영어학습자를 대상으로 3주간 실험 수업을 진행하였으며 그 결과를 정리하면 다음과 같다. 첫째, DALL-E2 활용 영어 작문 활동에 대한 설문조사 결과, 자신감, 흥미 및 인공지능 기반 도구 활용 쓰기에 대한 인식이 긍정적으로 변화하였음을 알 수 있었다. 아울러 통계 적으로 유의미한 차이가 나타났음을 확인하였으며 이는 인공지능 활용 학습이 영어 작문 및 전반적인 영어 학습에 있어 긍정적인 영향을 미쳤다는 것을 의미하였다. 둘째, DALL-E2 활용 영어 작문 활동에 대한 소감문을 내용분석을 통해 분석한 결과, 핵심 주제를 3가지(인지적, 정의적, 심동적 특성)로 추출할 수 있었으며 영어 학습에 있어 인공지 능 기반의 DALL-E2의 활용과 접목은 학습에 대한 새로운 흥미와 도전, 의지, 적극성을 높이며 궁극적으로는 영어표 현력(productive skill) 향상에 일부 기여한 것으로 해석할 수 있었다.

The purpose of this pilot study is to examine the responses of middle school students to English learning after conducting English writing activities using DALL-E2, an image-generating artificial intelligence tool. To this end, an experimental class was conducted for 3 weeks for 15 middle school English learners, and the results are summarized as follows. First, as a result of a survey on English writing activities using DALL-E2, it was found that confidence, interest, and awareness of writing using artificial intelligence-based tools changed positively. In addition, it was confirmed that there was a statistically significant difference, which meant that learning using artificial intelligence had a positive effect on English writing and overall English learning. Second, as a result of analyzing the English writing activities using DALL-E2, core themes could be extracted into three (cognitive, affective, and psychodynamic characteristics), and the use and implementation of artificial intelligence-based DALL-E2 in English learning showed potential to increase learning interest, challenge, will, and desire in learning and ultimately contribute to enhancing productive skill.