Earticle

In order to simplify and reduce the cost of the analysis process for reference samples, STR typing using direct PCR buffer was confirmed. To select an efficient buffer, STR typing was performed using two kinds of buffers. As a result of comparing the STR full profile detection rate and peak height through the heat treatment-based rapid extraction method of the reference samples, it was confirmed that STR GO! was more efficient than Prep-n-Go. In addition, when using commercially available STR and Y-STR analysis kits (GlobalFiler, PowerPlex Fusion, Y23), it was found that there was no problem in result analysis even if the PCR reaction volume for DNA extracted with STR GO! was reduced to 10 uL. However, the detection rate of STR profiles decreased in DNA that had passed time after extraction. To overcome this, the dilution ratio of the PCR amplification product was adjusted and it was confirmed that there was no problem with STR typing even 4 weeks after extraction. This study is expected to be of great help in simplifying and reducing costs of STR typing for reference samples.

본 논문은 서로 다른 두 구조의 캐쉬와 새로운 뱅크선별기를 이용하여, 보다 효율적인 뱅크관리 메커니즘을 응용한 새로운 개념의 캐쉬 구조에 대한 설명을 한다. 크기가 작음에도 불구하고, 낮은 접근 실패율(Miss ratio)와 높은 저전력 효과는 기존의 일반적인 직접사상 캐쉬와 비교했을 때, 성능면에서 월등한 차이를 나타내고 있다. 이러한 결과의 원인은 직접사상 캐쉬와 완전연관 버퍼의 최적화된 구성과. 효과적인 뱅크선별기를 사용하여 적은 전력에도 높은 성능을 발휘하는 새로운 메커니즘을 사용하였기 때문이다. 제안한 구조의 성능은 다양한 크기의 직접사상 캐쉬와 비교하였으며, 접근 실패율, 평균 메모리 접근 시간, 전력소비, Energy * Delay Product 등 모두 4가지의 지표를 사용하였다.