Earticle

Objectives : To confirm whether acupuncture can suppress the degree of seizure and the activation of astrocytes in hippocampi using kainic acid(KA)-induced epilepsy mouse model. Methods : 8 weeks C57BL/6 mice(20~25 g) were given acupuncture once a day at acupoint HT8(Shaofu) bilaterally during 2 days before KA injection. After an intraperitoneal injection of 30 mg/kg KA, acupuncture treatment was subsequently administered once more(total 3 times), and the degree of seizure was observed for 120 min. The neuronal cell death, pERK expression, and astrocyte activation confirmed 1 hour and 24 hours after KA injection. Results : Acupuncture treatment at HT8 suppressed KA-induced epileptic seizure. One hour after KA injection, the pERK expression was increased, which was reduced by the acupuncture treatment. Twenty four hours after injection, the treatment decreased the KA-induced neuronal cell death, the interleukin-$1{\beta}$ expression and the astrocyte activation in the CA3 region of the mouse hippocampus. Conclusions : Acupuncture treatment at HT8 decreases the KA-induced epileptic seizure, the neural cell inflammation and death.

벼의 염기서열 분석이 완료됨에 따라 유전자의 세포내 기능을 밝히기 위한 기능유전체 연구가 활발히 진행되고 있다. 이를 위해 효율적으로 아그로박테리움을 이용해 원하는 유전자를 식물체 내로 형질전환을 하기 위한 노력은 지금도 계속 진행되고 있다. 본 실험에서는 캘러스를 유기한 후 아그로박테리움을 이용해 접종하는 기존의 방법과 달리, 성숙 종자를 소독한 후 2,4-D가 포함된 액체배지에 24시간 침종하여 배 부분이 발아하기 시작하는 종자를 이용해 바로 아그로박테리움을 접종하여 체세포변이의 발생을 최소화하고 유전자를 포함하고 있는 아그로박테리움이 식물 조직내로 침투할 수 있는 효율을 증가시키며, 그 후 캘러스를 유기하여 재분화 시킴으로써 형질전환 식물체를 얻는 방법을 새롭게 수립하였다. 배양과정 중 공동배양 배지에 아그로박테리움 성장억제물질인 silver nitrate와 항산화 물질인 DTT를 첨가하여 공동 배양 기간을 7일 이상으로 늘림으로써 벼 형질전환효율을 증가시킬 수 있었고, PCR 분석을 통해 원하는 목표 유전자가 형질전환체에 안정적으로 도입이 되는 것도 확인할 수 있었다. 또한, 이 방법은 형질전환 효율이 낮은 일품벼와 같은 품종에도 적용할 수 있을 것으로 판단된다. 이러한 결과를 종합해 볼 때, 본 실험을 통해 얻어진 새로운 공동배양 방법은 우수한 농업적 형질을 가진 벼 육종 소재 및 품종 개발시 효율적으로 이용할 수 있을 것으로 생각된다.

Rice is the most important crop as a model plant for functional genomics of monocotyledons. Rice is usually transformed using Agrobacterium tumefaciens. However, the transformation efficiency using previous method is still low. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the inoculation and co-cultivation step. We directly inoculated Agrobacterium containing a CIPK15 gene under the control of CaMV 35S promoter and NOS terminator in the pCAM1300 vector into the pre-soaked seeds in N6D media for 24 hours. After 7 days of culture at $25^{\circ}C$, calli were formed on seeds cultured on the co-cultivation medium containing an antioxidant compound (1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (3 mg/L silver nitrate). We obtained 35 and 22 transgenic plants in rice cultivars, Gopumbyeo and Ilpumbyeo, with increase of transformation efficiency by 30.4% and 22.6%, respectively compared to the general transformation method. The new method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

Objective: This study was performed to compare knowledge of and expectations for traditional medicine between Korean, Chinese and Taiwanese, to figure out whether these factors influence the intention to use traditional medical treatment in the future. Methods: One-hundred ninety-nine Korean, eighty-five Chinese, and one-hundred seventy-four Taiwanese subjects responded to the survey. A one-way ANOVA was performed to compare experiences of traditional medical treatment, knowledge of both traditional medicine and western medicine, expectations for traditional medicine, and future intention to use traditional medical treatment. Multiple regression analyses were also performed to evaluate a possible correlation between the other factors and the intention to use traditional medical treatment in the future. Results: The experiences of traditional medical treatment were in the order of Taiwanese, Korean, and Chinese, from more frequent to less frequent. Chinese had more knowledge of traditional medicine compared to Taiwanese and Koreans, and the expectations for traditional medicine were in the order of Chinese, Korean and Taiwanese, from higher to lower. The intentions to use traditional medical treatment in the future were in the order of Korean, Chinese and Taiwanese, from more to less. Multiple regression analyses showed that experiences of traditional medical treatment, knowledge of traditional medicine, and expectations for traditional medicine were associated with the intention to use traditional medicine in the future in Korean and Taiwanese, but not in Chinese. Only the expectations for traditional medicine were associated with the future intentions to use traditional medical treatment in Chinese. Conclusions: These findings suggest that Korean and Taiwanese may need to improve their knowledge of traditional medicine whereas Chinese need to link their knowledge of traditional medicine with the intention to use it in the future. Understanding the characteristics of traditional medicine would probably contribute to enhancing the intentions to use it more.

This study explores the applicability of social capital theory in IT project management. Specifically, an empirical model is developed using different types of leadership competencies (emotional, cognitive, and social) as independent constructs impacting IT project performance. Social capital shared among team members are measured and placed as a mediating construct between leadership competencies and performance. Using PLS analysis of 120 data points collected through a survey of IT project participants in two large electronic manufacturers, direct and indirect impacts of leadership competencies are explicated. Research results reveal that emotional leadership competency seems to directly influence the project performance but not through team social capital, while social leadership competency seems to indirectly influence the project performance through team social capital but not directly onto the project performance. Cognitive leadership competency is the only leadership competency that maintains direct and indirect influence on project performance. Total effect of cognitive competency on project performance is almost two times larger than the total effect of emotional leadership competency and six times larger than the total effect of social leadership competency. Implications of findings are discussed at the end, and further studies are suggested.

Purpose: This study was conducted to compare the postural habits, body image, and peer attachment of adolescents with idiopathic scoliosis and healthy adolescents, and to examine the correlation between body image and peer attachment in each group. Methods: Eighty-three adolescents with idiopathic scoliosis and 83 healthy adolescents were recruited from 4 middle schools located in W city. Data were collected from November 22 to December 6 2010 using a structured questionnaire including items about postural habits, body image, and peer attachment. Data were analyzed using descriptive statistics, $X^2$-test, independent t-test and Pearson Correlation Coefficient. Results: Compared to healthy adolescents, adolescents with idiopathic scoliosis were more likely to use one hand, to lean back while seated, and to put weight on one leg while standing. The score for body image was significantly lower for adolescents with idiopathic scoliosis compared to healthy adolescents, but peer attachment was not significantly different between the two groups. Body image was significantly correlated with peer attachment only for the adolescents with idiopathic scoliosis. Conclusion: Interventions to promote balanced postural habits in adolescents with idiopathic scoliosis are needed. Information on body image and peer attachment for these adolescents should also be considered when developing interventions.

A study was undertaken to examine the antioxidative activity and the quality attributes of broccoli stem powder pound cakes. Broccoli stem powder was added to the batter at a ratio of 5, 10 and 15% respectively. The antioxidant activity was estimated by DPPH free radical scavenging activity, the total phenolic compounds content and flavonoid content in Broccoli stem powder and pounds cakes. Antioxidative activity was highly correlated with the total phenolic and total flavonoids contents of broccoli stem pound cakes respectively($r$=0.937, $r$=0.981). The effect of cake improver on quality characteristics of cake broccoli stem powder and pound cakes were estimated in terms of the specific load volume, hardness, hunter value of crumb and crust and sensory quality of cakes. The specific volume were increased significantly with increasing substitution level of cake improver and broccoli stem powder($p$ <0.01). The lightness were significantly decreased with increases in broccoli stem powder of pound cake crust and crumb($p$ <0.01, $p$ <0.01). The hardness, chewiness and gumminess and cohesiveness were tended to reduce in both of powder. The consumer acceptability score for 5~10% broccoli stem pound cakes ranked significantly($p$ <0.01) higher than those of the other groups in taste, flavor and overall preference. This results showed that cake improver & broccoli stem powder are a good ingredient to increase consumer acceptability and the healthy.

A study was undertaken to examine the effect of the addition of job's tears(Coixlachryma-jobi L.) chungkukjang(soybeanfermented food) and wheat bran powder on quality attributes of pound cakes. Job's tears chungkukjang and wheat bran powder was added to the batter at a ratio of 10, 12.5 and 15% respectively. The antioxidant activity was estimated by DPPH free radical scavening activity, the total phenolic compounds content and flavonoid content in job's tears chungkukjang & wheat bran powder and pounds cakes. Antioxidative activity was highly correlated with the total phenolic and total flavonoids contents of job's tears chungkukjang and wheat bran pound cakes respectively(r=0.9671, r=0.8335). The quality characteristics of job's tears chungkukjang powder and wheat bran powder pound cakes were estimated in terms of the specific load volume, hardness, hunter value of crumb and crust and sensory quality of cakes. The specific volume were increased significantly with increasing substitution level of job's tears chungkukjang and wheat bran powder(p<l0.01). The lightness were significantly decreased with increases in job's tears chungkukjang and wheat bran powder of pound cake crust and crumb(p<0.01, p<0.01). The hardness, chewiness and gumminess were tended to reduce, while cohesiveness with increase in both of powder. The consumer acceptability score for 10~15% job's tears chungkukjang and wheat bran powder pound cakes ranked significantly(p<0.01) higher than those of the other groups in taste, flavor and overall preference. This results showed that job's tears chungkukjang and wheat bran powder are a good ingredient to increase consumer acceptability and the healthy.

작은 크기 다양한 입출력 포트 등의 많은 이점으로 PCB 회로기판에서 볼그리드배열(BGA) 방식의 기판이 사용되고 있다 하지만 BGA 기판 내부 기포는 기판의 성능을 떨어뜨리고 실패를 야기한다 자동적으로 X-ray 영상에서 기포를 검출하기 위해서 본 논문에서는 국부 영상 명암 변량 측정 방법을 이용한 새로운 덩어리 검출기를 제안한다 국부 영상 명암 변량의 활용은 영상의 밝기 기포의 모양, 기포의 위치 및 부품간의 간섭등에도 연산 결과에 영향이 적은 불변성을 가진다. 또한 박스 필터의 다양한 사이즈를 적용하여 다양한 크기의 영상을 분석하므로 결과적으로 제안한 덩어리 검출 필터는 기포의 다양한 크기에 도강력하다. 실험 결과는 제안한 기법이 정상 부위를 비정상 부위 로보이는 확률false-ratio)을 낮게 유지하면서 기포 검출률을96.104% 까지 높일 수있음을 보인다.

Due to the advantages of small sizes, more I/O ports, etc., Ball Grid Array (BGA) has been used in the production of printed circuit board (PCB). However, BGA voids can degrade the performance of the board and cause failure. To automatically detect the voids in X-ray image, a novel blob filter that makes use of the local image gradient magnitude is proposed in this paper. The utilization of the local image gradient magnitude makes the proposed filter invariant to the image brightness, void shape, void position, and component interference. Furthermore, different sizes of box filters are employed to analyze the image in multi-scale, and as a result, the proposed blob filter is robust to void size. Experimental results show that the proposed method can obtain void detection accuracy up to 96.104% while keep low false ratio.

기존 보고된 바에 의하면 한국 자생 버뮤다그래스는 군집 내에서 형태학, 생육 특성, 세포학적 특성에 대해 유전적으로 매우 다양한 변이를 보여주었다. 버뮤다그래스의 염색체 수와 핵 DNA 량에 따르면 배수성 수준의 범위가, 3배 체(2n=3x), 4배체(2n=4x), 5배체(2n=5x), 6배체(2n=6x)로 나타났었다. 본 연구에서는 한국에서 휴면이 유도되는저온과 짧은 일장에 대한 항산화효소(superoxide dismutase, catalase, peroxidase, ascorbate peroxidase)의 다양한 반응과 각 버뮤다그래스 세포형의 세포막 안정성을 조사하였다. 모든 항산화효소는 휴면 기간동안 높게 나타났으나, 과산화수소를 물과 산소 분자로 변환시키는 헴기를 함유한 카탈라제는 6배체 버뮤다그래스를 제외한 세 개의 세포형에서 휴면이 개시되기 전에 활성화되었다. 상대적으로 세엽이며 생육속도가 빠른3배체와 4배체는 superoxide dismutase와 peroxidase 효소의 활성이 증가됨을 확인하였다. 수산기를 가진 라디칼에 의해 손상을 받은 세포막에서 지질과산화의 산물인 말론디알데히드(MDA)는 온도가 감소함에 따라 모든 세포형에서 증가되었고, 방어적인 항산화효소를 더 갖고 있는 3배체와 4배체는 MDA 생산이 현저하게 더 낮게 나타났다. 전해질 유출은 5배체와 6배체에서 더 높았던 것과 유사하게, 저온이 적용될 때 외견상으로 세포막에 더 손상을 받는 것 같았다. 실험 결과, 서로 다른 세포형(cytotype)의 항산화 반응은 유전적으로 특이적이며, 이는 버뮤다그래스에서 저온 저항성과의 연관성을 분자 수준에서 더 연구하는 것이 필요하다.

Korean bennudagrass collections showed diverse genetic variations in their morphology, growth habit, and cytological aspects. Chromosome number and nuclear DNA content of the bennudagrasses indicated a ploidy level ranging from triploid (2n=3x) to hexaploid (2n=6x). In this study, we investigated the different responses of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase) and cell membrane stability of those bennudagrass cytotypes to lower temperature and shorter day length, which meets a dormant induction in Korea. All the antioxidant enzymes were found to be higher during dormant stage, while the heme-containing catalase which converts hydrogen peroxide ($H_2O_2$) to water and oxygen molecules was activated before dormant initiation in the three cytotypes except for hexaploid bennudagrass. The triploid and tetraploid which exhibited relatively finer leaves and a rapid establishment speed were found to show increased activities of superoxide dismutase and peroxidase enzyme. The malondialdehyde(MDA) which is a product of lipid peroxidation in the cell membrane damaged by the hydroxyl radical was increased in all cytotypes as temperature declined, and tri- and tetraploids which had more protective antioxidant enzymes demonstrated a significantly lower MDA production. Similarly electrolyte leakage was higher in penta- and hexaploidy, seemingly more damage to cell membrane when low temperature was implemented. Results indicated that antioxidant responses of different cytotypes were genetically specific, which needs to be investigated the relevance with the low temperature tolerance in the bermudagrass further at the molecular level.

축산폐수 혐기소화 유출수 중의 생물학적 난분해성 유기물의 분해를 위하여 오존 기반의 고도산화 기술을 적용하였다. 배출수의 COD 및 색도는 각각 9200~9500 mg/L 및 0.384 (400 nm)이고 1/10 희석하여 실험에 사용하였다. 공급 오존은 버블의 크기가 $13{\mu}m$인 마이크로버블 오존과 $105{\mu}m$인 일반 오존버블과의 차이를 고찰하였다. 마이크로버블 오존을 사용함으로써 오존의 용해도와 라디칼 생성량이 증가되었고 일반 오존버블에 비하여 COD 및 색도의 제거효율이 각각 85% 및 26% 향상되었다. 마이크로버블을 포함한 $O_3/UV$, $O_3/H_2O_2$, $O_3/UV/H_2O_2$의 조합을 비교한 결과 오존 단독 처리에 비하여 색도 제거율이 5~10% 정도 증가되었으며, 오존에 비하여 UV나 $H_2O_2$의 색도제거에 대한 기여가 크지 않음을 알 수 있었다. 반면 COD에 대해서는 $O_3/UV/H_2O_2$ 적용시 오존 단독에 비하여 2배 이상 제거율이 증가하였으며 UV보다는 $H_2O_2$의 기여도가 더 컸다. 한편 마이크로 오존의 사용시 증가된 용존오존 및 라디칼 활성으로 인하여 오존 공급을 중단한 후에도 UV 또는 $H_2O_2$를 적용함으로써 추가적인 COD 분해 효과를 지속적으로 유지할 수 있었다.

Ozone-based advanced oxidation was applied for the treatment of anaerobic digestion effluent of livestock wastewater. Initial COD and color value were 930 mg/L and 0.04, respectively, and the 1/10-diluted wastewater was used for the study. The treatment characteristics were compared between the conventionally generated ozone ($105{\mu}m$) and microbubbled ozone ($13{\mu}m$). The use of microbubbled ozone improved the removal of chemical oxygen demand (COD) and color by 85% and 26%, respectively, compared with the conventionally bubbled ozone. The application of microbubbled $O_3/UV$, $O_3/H_2O_2$, $O_3/UV/H_2O_2$ combinations resulted in 5~10% higher color removal than ozone alone, which implies that the contribution of UV or $H_2O_2$ is not significant in color removal. On the other hand, COD removal could be increased two folds compared with ozone alone through $O_3/UV/H_2O_2$ combination. The contribution of $H_2O_2$ was bigger than UV for COD removal with microbubbled ozone. Due to the enhancement of dissolved ozone and radical activity, the microbubbling enabled us to additional COD removal even after stopping ozone supply in the presence of UV or $H_2O_2$.

율무청국장이 고지방식이를 섭취한 흰쥐의 체중 및 체지방, 혈청과 간, 분변의 지질농도 및 혈당농도 등에 미치는 영향을 조사하고자 SD계 수컷 흰쥐를 대상으로 고지방식이 (D12492, 45 kcal fat)에 청국장 및 율무청국장(율무 함량 20%) 분말을 각각 10%, 20%씩 중량비로 첨가하여 5주간 급여한 결과는 다음과 같다. 체중증가량은 군별 유의성이 없었으나 식이효율은 고지방식이 대조군에 비해 율무청국장 분말 20% 첨가군에서 유의적으로 낮았으며(p<0.05), 지방조직의 무게는 실험군 간에 유사한 수준을 나타내었다. 배변량 및 간 조직의 총콜레스테롤과 중성지방 함량은 군별 유사한 경향을 나타내었으며, 분변의 총콜레스테롤 함량은 군별 유의적 차이가 없었으나 중성지방 함량은 대조군에 비해 실험식이 첨가 수준에 따라 유사하거나 증가하는 경향을 보였다. 혈청 총콜레스테롤, HDL-콜레스테롤 및 LDL-콜레스테롤 농도는 군별 유의적인 차이가 없었으나 동맥경화지수와 심혈관위험지수는 청국장 분말 10%, 20% 및 율무청국장 분말 20% 첨가군에서 대조군보다 유의적으로 낮았으며(p<0.05), 중성지방 농도는 대조군에 비해 청국장 및 율무청국장 분말 20% 첨가군에서 유의적으로 감소하였다(p<0.05). 한편, 혈당 농도는 실험군 간에 유의적인 차이가 없는 것으로 드러났다. 이상에서 율무청국장 식이는 식이효율의 감소, 중성지방의 배설량 증가, 그리고 혈청 중성지방 농도의 감소 및 동맥경화지수와 심혈관위험지수의 감소 등의 효과를 확인할 수 있었다.

This study was performed to investigate the effects of Cheonggukjang added with Job's tears (CAJT) on antiobesity and lipid profiles on diet-induced obese rats. Male Sprague-Dawley rats were fed high-fat diet only (A, control), and high-fat diet with Cheonggukjang powder 10% (B), CAJT powder 10% (C), Cheonggukjang powder 20% (D), CAJT powder 20% (E) for 5 weeks. Body weight gains were similar in the experimental groups, but FER was significantly lower in the group E than group A (p<0.05), and adipose tissue weights in groups with Cheonggukjang and CAJT powder were similar to those in the group A. The lengths of small intestine were significantly longer in the groups B, C, D, and E than the group A (p<0.05). Hepatic concentrations of total cholesterol (TC) and triglyceride (TG) were similar to each other. Fecal weights were not significantly different among the groups. Fecal TC concentrations were similar in the experimental groups, but fecal TG concentrations were greatly increased in the groups B, D, and E (p<0.05). The serum TC, HDL-C and LDL-C levels varied slightly among the groups, but serum TG levels were decreased in the group D and E than in the group A (p<0.05). The AI and CRF were marginally decreased in the groups B, D, and E than in the group A (p<0.05). However, blood glucose levels were not significantly different among the groups. From the findings, it had shown that Job's tears Cheonggukjang could improve lipid profiles.

Objectives : The purpose of this study is to develop an apparatus which can take a facial image by self-operated capturing technique. The user can obtain one's facial image immediately after adjusting facial tilt and focusing distance. The system has been designed for classifying Sasang typology based on facial image. Methods : The system is composed of a Webcam, one-way glass mirror and mini LCD. The Webcam takes a facial image which is displayed on the mini LCD. Then the user can see and adjust to the right position in the real time through the image mirror-reflected from the mini LCD. The optical sensor is used to estimate the proper focusing distance. To verify the performance of the system, 11 characteristic points on the facial image are used and compared with high performance DSLR camera(D700) by applying the coefficient of variance and Bland-Altman Plot. Results : The developed system and D700 show enough agreement with the small coefficient of variance to analyse constitutional types with a facial im mage. However, the result of Bland-Altman plot shows that the width parameters have distortions owing to short focusing distance. Conclusions : The system is expected to be utilized on u-healthcare services for home environment after improving the distortion in the width parameters.

스텔스 기능성 섬유의 위장은 주간의 경우, 육안 및 망원경 관측에 의해 결정되며 주변자연환경 color matching과 패턴이 핵심 스텔스 요인으로 구성되고, 가시광선 영역 스텔스로 표현할 수 있으며, 군 위장 체계에 있어 기본이 되는 기술 분야로 모든 군 위장제품에 활용되는 필수적인 기술 분야이다. 따라서 가시영역의 위장은 다양한 색상의 위장포와 포의 펀칭 등에 의한 파형특성을 부여하고 주위환경과 특성 조화를 이룸으로써 대상을 위장한다. 주요인자는 색상별 색도, 색차이다. 야간 위장은 근적외선 관측 장비의 탐지에 의해 결정되며 주변자연환경 NIR 반사율과 위장제품의 NIR 반사율 저하/제어기술이 핵심 스텔스 요인으로 구성되고, 근적외선(NIR) 영역 스텔스로 표시할 수 있으며, NIR 스텔스는 대개의 경우 기본적으로 섬유제품이 NIR 영역에서 높은 반사율을 나타내므로 NIR 반사율 저하/제어기술이 핵심이라 할 수 있다. 따라서 근적외선 영역(600~1250nm)의 적외선 반사특성을 산림지역의 반사특성과 조화시켜 위장효과를 부여한다. 위장포에서는 적외선 흡수 색소를 사용하여 적절한 반사특성을 나타나게 해야 한다. 위장용섬유가 detector의 탐지에서 벗어나려면 현재 700~1250nm의 근적외선파장영역에서 주변환경과 유사한 반사율을 지녀야한다. 이에 본 연구에서는 "숲 연구소" 및 서울대학교 "지반공학연구실"의 자문을 받아서 우리나라에서 가장 많이 존재하는 자연환경시료를 선정하여, 선정된 시료의 근적외선영역에서의 반사율을 분석하였다. 자연시료는 산간, 해안, 평야지형으로 각각 구분하여 주변 자연지형에 따른 자연시료의 근적외선영역의 반사율값을 비교하였다. 북한산에서 산간지형의 시료를 채취하였고, 해안지형의 시료는 강화도 동막해수욕장 부근, 평야지형의 시료는 인천 강화도 평야지역에서 채취하여 분석하였다. 비교분석한 지형별 자연시료의 근적외선 반사율값의 데이터베이스구축을 통하여 기존의 위장복과의 비교 적용 및 차후 개선사항 등을 검토하고자 한다.

율무의 첨가가 청국장 냄새 저감에 영향을 미치는지 확인하기 위해 대두와 율무의 혼합 비율별 가공 적성 조사 및 적정 배합비 설정, 이에 따른 관능특성 평가, 향기 패턴 및 성분 분석을 실시하였다. 대두만 사용된 대조구와, 대두와 율무를 각각 1:1, 2:1, 3:1, 4:1의 비율로 혼합한 처리구에 Bacillus subtilis 종균을 접종하여 발효시킨 청국장과 재래식 청국장을 실험에 사용하였다. 율무청국장의 열량, 지방, 단백질, 회분, 아미노태 질소의 함량이 대조구보다 낮았으며, 수분과 탄수화물은 높았다. pH는 율무청국장이 상대적으로 낮았고, 색도는 율무의 함량이 높을수록 백색도와 황색도는 높고 적색도는 낮은 경향을 보였다. 점질물 함량은 율무청국장에서 높은 경향을 보였고, 점질물 중의 glutamic acid 함량은 율무청국장이 상대적으로 낮았고 fructose 함량은 상대적으로 높았다. 관능평가 결과 대두와 율무 4:1 처리구의 기호도가 가장 높았다. 대조구와 율무청국장의 향기패턴은 재래식 청국장과 확연하게 달랐으며, 두 처리구는 비슷한 패턴이지만 구별이 가능하였다. 또한 대조구보다 율무청국장에서 pyrazine류 화합물이 적게 동정되어 청국장 냄새가 적게 난다는 것을 확인하였고, 냄새의 강도 관능평가 결과도 율무청국장이 유의적으로 낮았다. 이상에서 율무청국장을 제조할 때 율무의 함량은 20%를 첨가하는 것이 가장 적합하며, 율무청국장은 일반 청국장에 비해 냄새 저감 효과가 있음을 확인할 수 있었다.

This study was performed to develop an odor-reduced Korean traditional Cheonggukjang (fermented soybean paste) by adding Job's tears to Cheonggukjang made with soybean (control) only. The study included the determination of best addition percentage of Job's tears versus soybean, sensory evaluation, and aroma patterns and its components as well as proximate composition analyses of Cheonggukjang. Job's tears Cheonggukjang (CAJT) was prepared by inoculating Bacillus subtilis and fermented at $40^{\circ}C$ for 48 hours. Ratios of soybean : Job's tears for Job's tears Cheonggukjang were 1:1, 2:1, 3:1, and 4:1, respectively. In comparison of proximal composition, Job's tears Cheonggukjang was high in moisture and carbohydrates, but low in calorie, fat, protein, ash and amino nitrogen. The pH of CAJT was lower than those of control and conventional Cheonggukjang (CC) sold in the market, and the acidity was the lowest in CC. The color of Job's tears Cheonggukjang appeared the whiter and more yellowish, but less reddish in proportion to contents of Job's tears. The contents of viscous substances were higher in CAJT compared with those of control and CC. The contents of glutamic acid, the major components of viscous substances were lower in CAJT than in control and CC, and the contents of fructose higher in CAJT. The scores of sensory evaluation were the highest in Job's tears Cheonggukjang mixed with soybean : Job's tears=4:1. The aroma pattern of CC analyzed was conspicuously dissimilar to the control and CAJT and also discriminated by electronic nose examination. The pyrazines, volatile compounds peculiar to Cheonggukjang, were found to be lower in CAJT by SPME-GC/MS assay. Additionally, the acetic acid, butanoic acid, and naphthalene causing off-flavor were identified in CC, but not in Job's tears Cheonggukjang. The strength of odor through sensory evaluation was by far the lowest in CAJT among the groups. From the findings, it had shown that Job's tears Cheonggukjang would be produced successfully when the ratio of 4:1 (soybean : Job's tears) is employed. Also, it was proved that appropriate ratio of Job's tears addition makes Cheonggukjang odor-reduced and well accepted by people.

Objectives : The aims of this study were to evaluate a blister caused by cupping. Methods : We searched relevant case reports, survey, and review articles using databases of online bibliography. Results : 1. The fluid in the blister caused by cupping therapy is normal substance by laboratory analysis. The fluid has no signs of infection in the culture, Gram stain, or tissue biopsy 2. In histological finding, the blister caused by cupping therapy is made by dermo-epidermal seperation at subcellular level. Suction blistering was neither inflammatory nor autolysis activation of lysosomal hydrolases. 3. Blistering times directly, related to suction pressure. Suction blister formation time is accelerated in older subjects compared with younger individuals and higher temperature was more susceptable to the blister compared with lower temperature. The flexor aspect of forearm is a easy site for suction blister formation compared with leg and abdominal site. 4. Blister caused by cupping therapy is treated by regular and judicious changes of sterile dressing over several weeks. The vesicles healed well and left no visible scar. Conclusions : Blister caused by cupping therapy is artificially controlled by doctor's therapeutic purpose. Blister is not histologically injurious to health and the blister is a natural concomitant after cupping therapy.

Job's tears(Coix lachryma-jobi L.) chungkukjang powder and wheat bran powder were added to American cookies for practical use as healthy compounds. We examined the antioxidant activity and quality characteristics of cookies prepared with different amounts(as ratios of 10%, 20%, 25% to the flour quantity) of Job's tears chungkukjang & wheat bran powder. The antioxidant activity was estimated by DPPH free radical scavenging activity, the total phenolic compounds content and flavonoid content in Job's tears chungkukjang & wheat bran powder and cookies. The quality characteristics of Job's tears chungkukjang & wheat bran powder American cookie were estimated in terms of bulk density, pH of the dough, spread ratio, color, texture profile analysis, proximate composition, and sensory evaluations. While the spread ratio and the total polyphenol contents, flavonoid contents and DPPH free radical scavenging activity of cookies significantly increased, pH, hardness and L value of the cookies decreased with increasing Job's tears chungkukjang & wheat bran powder(p<0.01). The consumer acceptability score for the 10~20% Job's tears chungkukjang & wheat bran powder American cookie ranked significantly(p<0.05) higher than those of the other groups in texture andoverall preference. This suggests that Job's tears chungkukjang and wheat bran powder are good ingredient candidates for increasing consumer acceptability and functionality of cookies.

1. Objectives: We report the relation between the questionnaire and the health state on the Sasang Constitution. 2. Methods: We carried out a clinical test, in which 2 Oriental medical doctors participated to diagnose health state in each Sasang Constitution with 239 subjects. The subjects were divided into 3 subgroups, which are allocated to healthy group, poor health group and disease group. 3. Results: We extracted 4 questions for Taeeumin, 5 questions for Soyangin and 3 questions for Soeumin from the whole 98 questions respectively. Soyangin and Taeeumin share a same question that shows significant difference by health status. For the statistical analysis, we used a Chi-square test. As a result, we found out that Taeeumin showed different distributions of answers in direct/indirect characteristic, perspiration, frequent urination, regular defecation. According to the health status Soyangin showed the different distribution of the answers in color of urination, frequent urination, mouth dryness, abnormal perspiration, discomfort of head. Soeumin showed the different distribution of the answers in cold and chilly feel of abdomen, digestion status and cause of digestion problems when body condition gets worse. 4. Conclusions: This is the first work which reports the relatedness between Questionnaire and Health State in each Sasang Constitutions by an objective clinical test. We found out that several questions have different distributions by health status in each Sasang Constitution. These extracted questions are categorized not only by Wansilmoobyung(완실무병), but also by other symptoms and body status.

Objectives : To understand the strength and weakness of traditional warm needle acupuncture based on existing research outcomes in Korea mostly and to suggest how to build the desirable warm needle acupuncture apparatus by overcoming demerits of traditional and currently existing ones. Methods : We searched warm needle relating papers in Korean with the key words of '온침, 화침, 열자극' by using DBpia, Journal of Korean Oriental Medical Society, Journal of Korean Acupuncture & Moxibustion Society and Journal of Society for Meridian and Acupoint. Degree dissertations in Korean were also searched with the same words through the search engine of Library of Kyung Hee University. We also searched with words of 'warm, thermal, heat, needle, acupuncture, temperature' via Pubmed and found a small number of English written papers and large number of Chinese written ones. To find english translated version of those papers, we googled with the same words with no success. Results : About 20 papers on warm needle acupuncture written in Korean were found and analyzed with respect to experimental factors that affected the thermal properties or the amount of heat stimulus of the acupuncture. More rigorous descriptions seemed to be required on the insertion depth, duration and manipulation of warm needle treatment. A basic heat transfer model was presented for the clarification of heat loss through the needle of warm needle acupuncture. Environmental factors such as air flow on the properties seems to be considered for the warm needle acupuncture. Conclusions : Papers on warm needle acupuncture were reviewed and analyzed based on their thermal properties and tools such as needles and moxa cones. Several suggestions were made on the descriptions relating the properties. Necessary specifications were introduced to aim modernized warm needle systems.

본 논문에서는 세 집단만을 판별분석 할 경우에 계산되는 오분류확률에 영향을 미치는 이상치 판별을 목적으로 하며, 쉽게 응용 가능한 간단한 영향함수식을 제시하였다. 그리고 제시된 수식을 이용하여 안면 데이터로 세 가지 사상체질을 분류해보고 각 관찰값들의 오분류확률에 대한 영향함수를 계산하였다. 이상치를 제거하고 재 판별분석을 하는 데 있어, 오분류확률에 대한 영향함수를 이용하는 것이 효율적인 방법임을 확인하였다.

The influence function is used to develop criteria to detect outliers in discriminant analysis. We derive the influence function of observations that estimate the the misclassification probability in discriminant analysis for three groups. The proposed measures are applied to the facial image data to define outliers and redo the discriminant analysis excluding the outliers. The study proves that the derived influence function is more efficient than using the discriminant probability approach.