We propose a mediator-free glucose biosensor which use immobilized glucose oxidase on tin oxide. Direct electron transfer is possible, because the tin oxide shows redox properties similar to those of the mediators. The preparation method for tin oxide and glucose oxidase-modified tin oxide is also simpler than the existing methods. Tin oxide provides an efficient surface for the immobilization of large amounts of enzymes, due primarily to the porosity of the surface. Glucose oxidase was immobilized on tin oxide via the entrapment method, using nafion. The suggested method provides a simple process for enzyme electrode fabrication. Glucose oxidase immobilized on tin oxide prepared in accordance with this method has a relatively large current response, as compared to those of other glucose biosensors. The sensitivity of the biosensor was 39A/mM, and a linear response was observed between 0mM to 3mM glucose. The Michaelis-Menten constant was determined to be lower than in electrodes prepared via the methods in current use. Also, this biosensor shows good reproducibility and stability.
저자
Yang Hee Kim [ School of Chemical and Biological Engineering, Seoul National University ]
Andrew Seong [ Taejon Christian International School ]
Young Je Yoo [ School of Chemical and Biological Engineering, Seoul National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동