Quantitative analysis of DNA microarray is one of important issues in mRNA expression profiling and estimation for infectious doses of pathogens1). Here, we newly introduced an artificial standard probe strategy for quantitative data analysis of pathogen detection oligonucleotide chip as a model system improved from our previous report2). The artificial standard probe was designed to have below 85% similarity with any bacteria in order not to occur non-specific hybridizations with other targets. Based on fluorescence intensities of artificial standard spots, we successfully demonstrated that raw fluorescence intensities of specific probe spots could be corrected to have quantitative correlations with target concentrations. Therefore, our suggested rtificial standard probe strategy may be used for correction of chip-to-chip variation and making possible quantitative analysis of one-color oligonucleotide microarray experiment.
키워드
quantitative analysisDNA microarrayartificial standard probe
저자
Byeong Hee Hwang [ Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) ]
Hyung Joon Cha [ Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동