Bo-rahm Lee, Tae Seok Sim, Sang-Myung Lee, Hyo-Jin Yoon, Yoon-Sik Lee, Yong-Kweon Kim, Byung Gee Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99994
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
We develop a micro analysis and detection system for replicase of the hepatitis C virus. The fabricated analysis system consists of a bead based microchip and a computer based controller. A microheater, a micro temperature sensor and a microreator for bead packing is integrated in one microchip to realize several sequences for the capture and the elution of replicase. In this study, we used the PEG-grafted PS bead on which RNA aptamers are immobilized and performed thermal denaturation for releasing target molecules, and analyzed by MALDI-TOF MS. We performed several experiments to optimize conditions. As a result, we could acquire several matched peaks (26 % sequence coverage) of HCV replicase using the following condition: at room temperature for 20 min of binding time and at 85℃ for 30s of thermal denaturation time.
저자
Bo-rahm Lee [ School of Chemical and Biological Engineering, Seoul National University ]
Tae Seok Sim [ School of Electrical Engineering and Computer Science, Seoul National University ]
Sang-Myung Lee [ School of Chemical and Biological Engineering, Seoul National University ]
Hyo-Jin Yoon [ School of Chemical and Biological Engineering, Seoul National University ]
Yoon-Sik Lee [ School of Chemical and Biological Engineering, Seoul National University ]
Yong-Kweon Kim [ School of Electrical Engineering and Computer Science, Seoul National University ]
Byung Gee Kim [ School of Chemical and Biological Engineering, Seoul National University, Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동