Earticle

초록

영어

Protein patterning is crucial for the development of protein chip and biosensors. The most important point of this technique is how to bind both protein and surface. A lot of studies were reported about this, for example protein immobilization on aminosilane or poly-L-lysine functionalized surface. However theses conventional methods have problems that strong binding by covalent bonding cause deformation of three dimensional structures of protein and by electrostatic force by poly-l-lysine mono-layered surface have a weak binding force for effective patterning. In this study, we have introduced the development of protein micro-patterning
on polyelectrolyte multilayer thin films(PEM) by consecutive layer-by-layer adsorption of polyelectrolyte via self-assembly technique. The property of PEM surface provided periodic oscillations in contact angle, which indicated the multilayer were correctly terminated by polycation and polyanion, respectively. Protein was immobilized on PEM by electrostatic interaction. As a model system, the printing of the fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA: 1 μg/ml) was performed. It showed good linearity over a range from 100 ng/ml to 255g/ml, indicating the feasibility of a quantitative measurement of concentration of target proteins in sample. The protein patterning fabricated on PEM provides higher signal intensity than patterning by conventional method. This novel method is well suited for immobilizing many types of biological molecules so that a wide variety of micro patterning formats can be developed.

저자

• Ji-Hye Lee [ Department of Chemical Engineering, Chungnam National University ]
• Hyun-Woo Shim [ Department of Chemical Engineering, Chungnam National University, ]
• Chang-Soo Lee [ Department of Chemical Engineering, Chungnam National University, ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576