Earticle

초록

영어

Extracellular production of recombinant proteins in E. coli has received considerable attention due to its significant advantages over cytoplasmic or periplasmic production. However, efficient secretion of recombinant proteins into the culture medium of E. coli remains a challenge due to the intrinsic limitations of the secretion machinery. Here we report a systematic proteome-based
approach for high-level extracellular production of recombinant proteins. First, the extracellular proteome of an E. coli B strain was analyzed to identify motifs as potential fusion partners. Next, we expressed each open reading frame of the selected motifs and determined the protein profiles of the culture medium. The highest secreting motif was used as the carrier protein to produce several "passenger proteins" in the culture medium. Those model passenger proteins
show a wide versatility with respect to the proteins' length and origin. In addition, the polypeptides accumulated in the medium at high concentrations ranging from 15 to 500 mg/L. These findings demonstrate a proteome-based approach for high-level production of recombinant proteins in the culture medium of E. coli.

키워드

저자

• Zhi-Gang Qian [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) ]
• Xiao-Xia Xia [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) ]
• Jong Hyun Choi [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) ]
• Sang Yup Lee [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Department of Bio and Brain Engineering, and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576