Hoai Thi Nguyen, Soo-Jung Gong, Yu-Mi Ha, Sung-Koo Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99985
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원문정보
초록
영어
White Spot Syndrome Virus (WSSV) occurs worldwide and causes up to 100% mortality within 7-10 days after the infection in commercial shrimp farm, resulting in large economic losses to the industry 1). Besides the economic impact of the disease, the natural aquatic ecology is also threatened as a wide host range of WSSV among crustacean 2). WSSV contains at least five major structural protein and the VP28 is a major component of its envelope protein. Of the viral structural protein, the envelope proteins usually play very important roles in virus infection and assembly. So the aim of this study is creating VP28 polyclonal antiserum and determining its WSSV neutralization ability for development of vaccines against WSSV. The VP28 ORF was successfully cloned in pCold I vector and expressed in E.coli BL21 . The purified VP28 protein was injected 3 times into a rabbit to produce VP28 polyclonal antibody. The polyclonal antibody was confirmed by ELISA and the antiserum was prepared for the neutralization. To study the role of VP28 in WSSV infection in Penaeus chinensis shrimp, an in vivo neutralization test was performed. The result of this experiment showed that WSSV infection was neutralizated by the VP28 polyclonal antiserum and that VP28 is involved in the infection process of WSSV. In further work, VP28 recombinant protein will be used for the production of vaccine against WSSV.
저자
Hoai Thi Nguyen [ Department of Biotechnology and Bioengineering, Pukyong National University ]
Soo-Jung Gong [ Department of Biotechnology and Bioengineering, Pukyong National University ]
Yu-Mi Ha [ Department of Biotechnology and Bioengineering, Pukyong National University ]
Sung-Koo Kim [ Department of Biotechnology and Bioengineering, Pukyong National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동