Quantum Dots-oligonucleotide conjugates, which are complementary to small region of target DNA, can probe interest microorganism in water environment. Luminescent Quantum Dots (QD) has narrower emission spectra and a continuous broad-band excitation compared with conventional fluorescent dyes (such as FITC and Cy3) so it has been used for many years for biological staining and diagnostics.1) It is easy to make QD-oligonucleotide conjugates by incubating QD coated with streptavidin and oligonucleotides modified with biotin. After incubation reaction free oligonucleotides, the unbound with QD, have to be removed from the mixture because hybridization efficiency decreases if unbound (unconjugated) oligonucleotides coexist with QD-oligonucleotide conjugates in the hybridization reaction. This study represents the application of field flow fractionation (FFF) for enabling easy separation of QD-oligonucleotide conjugates, which are required for effective-hybridizing to complementary target sequences. The FFF is one of the most versatile families of separation technique known. It has been successfully applied to studies of the environmental colloids, bacteria, proteins, and etc in a wide size range.2)3) The oligonucleotide used here is specific for Microcystis sp. to be able to produce microcystin and purified QD-oligonucleotide conjugates was applied to detect Microcystis sp. in the environmental sample.
저자
Jinwook Lee [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]
Sungyun Lee [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]
Jaeweon Cho [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]
In S. Kim [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]
corresponding author
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동