Earticle

초록

영어

Cell-free protein synthesis is a versatile tool for the rapid and parallel translation of genetic information into protein molecules1. However, the potential of this technology can only be fully exploited when protein molecules can be produced at high productivity and low cost. This study focuses on the development of a cost-effective and highly productive cell-free protein synthesis
system derived from Escherichia coli. We developed a new scheme for supplying energy using dual energy sources to enhance the regeneration of ATP and lower the rate of phosphate accumulation2. In the proposed scheme, where creatine phosphate and glucose were
simultaneously used as the energy sources, the phosphate released from the creatine phosphate was subsequently used in the glycolytic pathway for the utilization of the glucose, which enhanced the ATP supply and reduced the rate of inorganic phosphate accumulation. When tested against different proteins, the developed method produced 2-3 times more protein than the conventional ATP regeneration methods using single energy sources.

저자

• Tae-Wan Kim [ Department of Fine Chemical Engineering and Chemistry, Chungnam National University ]
• Ho-Cheol Kim [ Department of Fine Chemical Engineering and Chemistry, Chungnam National University ]
• In-Seok Oh [ School of Chemical and Biological Engineering, College of Engineering, Seoul National University ]
• Jin-Ho Ahn [ Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University ]
• Cha-Yong Choi [ Department of Fine Chemical Engineering and Chemistry, Chungnam National University, School of Chemical and Biological Engineering, College of Engineering, Seoul National University ]
• Dong-Myung Kim [ Department of Fine Chemical Engineering and Chemistry, Chungnam National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576