Earticle

초록

영어

Laboratory evolution has become a commonly used approach to address many of the fundamental long-standing questions about evolution as well as to provide an experimental basis for delineating the mechanistic basis of adaptive evolution, such as antibiotic resistance. We have got 7 independent glycerol evolved strains from wild type E. coli K-12 MG1655 by laboratory evolution. We have fully resequenced 5 independent glycerol evolved strains using the microarray-based Comparative Genome Sequencing service provided by Nimblegen Systems Inc. Surprisingly, one of them has only three mutations of whole genome in the endpoint. We added two mutations out of them into the wild type strain by site-directed mutagenesis. The double knock-in mutant, which had mutations in glpK and rpoC, had similar specific growth rate of the evolved strain. The two mutations thus seem to fully be responsible for the growth rate change during adaptive evolution. For intracellular metabolome analysis, we used capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The double knock-in mutant and the evolved endpoint strain showed very similar intracellular metabolite profile at mid exponential phase. Especially, concentrations of N-Carbamoyl-L-aspartate and N-Acetyl-L-aspartate, which participate in alanine and aspartate metabolism, were significantly decreased.

저자

• Baek-Seok Lee [ Institute for Advanced Biosciences, Keio University ]
• Tomoyoshi Soga [ Institute for Advanced Biosciences, Keio University ]
• Masaru Tomita [ Institute for Advanced Biosciences, Keio University ]
• Bernhard O. Palsson [ Institute for Advanced Biosciences, Keio University, Department of Bioengineering, University of California San Diego ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576