A bacterial strain isolated from soil for its potential to control fungal plant pathogens was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30°C. Biosurfactant lipopeptides produced by Bacillus subtilis CMB32 were precipitated by adjusting to pH 2 and extracting using chloroform/methanol, and then were purified using column chromatography and reversed-phase HPLC. Molecular masses of the lipopeptides were estimated by MALDI-TOF mass spectrometry as (a) 1080, (b) 1486, and (c) 1044 Da, respectively. They had cyclic structures and amino acid compositions of (a) Pro, Asx, Ser, Tyr, Glx, (b) Glx, Tyr, Thr, Ala, Pro, Ile, and (c) Glx, Leu, Val, Asx, respectively. Further analysis revealed that Bacillus subtilis CMB32 produced three antifungal lipopeptides: (a) iturin A, (b) fengycin, and (c) surfactin A.
저자
Pyoung Il Kim [ School of Chemical and Biological Engineering Institute of Molecular Biology and Genetics, Seoul National University, ]
Jaewon Ryu [ School of Biological Sciences and Technology and Biotechnology Research Institute, Chonnam National University ]
Youn-Tae Chi [ School of Biological Sciences and Technology and Biotechnology Research Institute, Chonnam National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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