Hemicellulose or cellulose-based biomass is the most abundant renewable resource in nature and composed of lignin and carbohydrate polymers containing glucose, xylose, arabinose, mannose and so on. As xylose is one of the major carbohydrates in hemicellulosic biomass, it becomes an attractive feed stock for the production of value-added compounds. Bioethanol is designated as ethanol produced from renewable biomass and can be used as an alternative fuel for automobiles. Microbial production of bioethanol from hemicellulose has been developed using bacteria and yeasts. S. cerevisiae , a traditional alcohol fermenting yeast, is unable to use xylose as a sole carbon source because of its deficiency in xylose metabolic enzymes including xylose specific transporter, xylose reductase and xylitol dehydrogenase. In this study, the xylose transport mechanism of recombinant S. cerevisiae was elucidated in kinetic and molecular levels. The zero trans -influx assays of glucose and xylose under high and low glucose conditions, and complementation experiments with sugar transporters were chosen for the investigation of xylose transport characteristics. In addition, the expression level of xylulokinase, the third enzyme in xylose metabolism, was modulated for the optimization of bioethanol production in recombinant S. cerevisiae .
저자
Yong-Cheol Park [ Center for Agricultural Biomaterials ]
Jin-Ho Seo [ Department of Agricultural Biotechnology, Seoul National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동