Earticle

초록

영어

As the amount of genetic information exponentially increases due to recent advances in genome sequencing, the demand for a faster and more parallel expression system has become greater than ever. For this reason, cell-free protein synthesis has received renewed attention as an alternative method for high-throughput gene expression. In contrast to in vivo gene expression methods, where protein synthesis is carried out in the context of cell physiology and is surrounded by cell walls and membranes, cell-free protein synthesis provides a completely open system that allows direct access to the reaction conditions. At the same time, most of the cell functions other than protein synthesis need not be maintained in the cell-free system. Here we present our recent achievements in developing a highly efficient cell-free protein expression system. Through a series of systematic approaches to address the key issues affecting the efficiency of cell-free protein synthesis reactions, currently we can produce milligram quantities of proteins in a simple batch reaction. Based on this effective and robust platform for rapid production of recombinant proteins, we currently are seeking to combine the techniques of cell-free protein synthesis with numerous other disciplines.

저자

• Dong-Myung Kim [ Department of Fine Chemical Engineering and Chemistry, Chungnam National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576