Earticle

초록

영어

The L-valine producing strain of Escherichiacoli was constructed by rational metabolic engineering and stepwise improvement based on transcriptome analysis and insilico gene knock-out simulation. The engineered L-valin producing base strain harboring pKKilvBN produced 1.31 g/liter L-valine. Comparative transcriptome profiling combined with in silico geneknock-out simulation was used for the enhanced production of L-valine. The VAMF strain (Val ΔaceF Δmdh ΔpfkA) harboring pKBRilvBNCED and pTrc184ygaZHlrp was able to produce 7.55 g/liter L-valine from 20 g/liter glucose, resulting in a high yield of 0.378 g L-valine per g glucose. The approaches described here can be a good example of developing strains for the enhanced production of amino acids based on systems biotechnology. [This work was supported by the Korean Systems Biology Project of the Ministry of Science and Technology (M10309020000-03B5002-00000). Further supports by the LG Chem Chair Professorship and KOSEF through the CUPS are appreciated].

저자

• Jin Hwan Park [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, Korea Advanced Institute of Science and Technology (KAIST) ]
• Kwang Ho Lee [ R & D Center for Bioproducts, CJ Corp. ]
• Tae Yong Kim [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, Korea Advanced Institute of Science and Technology (KAIST) ]
• SangYup Lee [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, Korea Advanced Institute of Science and Technology (KAIST), Department of BioSystems and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology (KAIST) ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576