We report a novel method to fabricate poly(vinyl alcohol) (PVA) hydrogel nanopatterns for constructing the nanoarray of lipid rafts as a form of tethered bilayer lipid membranes (tBLMs). Nanopatterns of UV-curable PVA were fabricated on gold substrates by ultraviolet nanoimprint lithography (UV-NIL). The surface of PVA hydrogel strongly restricted the adhesion of vesicles, resulting in selective arrays into the nanowells (NWs) of PVA hydrogel. A vesicle fusion and a mixed self-assembled monolayer (SAM) assisted vesicle fusion methods were used to confine the tBLM-formed lipid rafts into the nanopatterned PVA hydrogel. The tBLMs were not formed into the PVA hydrogel NWs by a simple vesicle fusion method. Nanoarray of tBLM-formed lipid rafts into PVA NWs has been accomplished by raft vesicle fusion onto a mixed SAM comprised of a mixture of 2-mercaptoethanol and a PEG lipopolymer. We expect that nanoarrayed lipid raft as a form of tBLMs provides an alternative platform for the analysis of transmembrane proteins, membrane-associated proteins, cell adhesion and a useful tool enabling the high-throughput applications such as nanodevices or nanochips.
Bong Kuk Lee [ The Institute of Scientific and Industrial Research, Osaka University, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency ]
Hea Yeon Lee [ The Institute of Scientific and Industrial Research, Osaka University, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency ]
Tomoji Kawai [ The Institute of Scientific and Industrial Research, Osaka University, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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