Earticle

초록

영어

L-Cysteine is often used in drug, cosmetics and food industries. The amino acid has been usually produced via acid-hydrolysis of hairs from animals or human. Since there are some problems with chemical methods, such as environmental pollutions and low yield, biological methods are to be developed. There have been reports about biological productions of L-cysteine1),2). However, the substrate concentration levels were so low that the industrial process of L-cysteine production could be not accomplished. In this study, D,L-2-amino-Δ
2-thiazoline-4-carboxylic acid (D,L-ATC) was used as the starting substrate of enzymatic reactions for L-cysteine productions. The eutectic melting technique was applied to prepare extremely high concentrated D,L-ATC solutions. Various salts were added for both enzyme stability and formation of eutectic mixtures. Among them, magnesium chloride and sodium carbonate were effective, leading to a eutectic mixture of 2.6 M. And the conversion of D,L-ATC to L-cysteine at ultra-high substrate concentration levels was performed using the whole cells of
Zoogloea ramigera as an enzyme source. Approximately 75% conversion was achieved at a substrate level of 0.5 M.

저자

• Sung Hun Youn [ Department of Biotechnology, College of Engineering, Yonsei University ]
• Hyun Jung Kim [ Department of Biotechnology, College of Engineering, Yonsei University ]
• Eun Ji Lee [ Department of Biotechnology, College of Engineering, Yonsei University ]
• Ha Na Park [ Department of Biotechnology, College of Engineering, Yonsei University ]
• Soo Hyun Woo [ Department of Biotechnology, College of Engineering, Yonsei University ]
• Yea Hoon Choi [ Department of Biotechnology, College of Engineering, Yonsei University ]
• Chul Soo Shin [ Department of Biotechnology, College of Engineering, Yonsei University ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576