Jae-Hoon LEE, Hyun-Seok KIM, Keum Sook CHOI, Sun-Young BAEK, Young Lim KIM, Won-Sang HWANG, Haeng Suk CHOI, Seung-Chul KIM, Chan-Wha KIM, Sook-Jin HUR
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99782
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
The high-risk type HPV is the main etiological factor of cervical cancer, which is one of the leading causes of cancer deaths in women worldwide, Therefore, identification of HPV type is very important and necessary for the diagnosis and remedy of cervical cancer1-3). In this paper, we determined HPV genotypes from the patient samples, which were confirmed as Pap-smear positive for HPV infection. To identify genotypes, we performed PCR/sequencing analysis by using the four different primer sets, PGMY, G4, G6 and GP1. At the same time, β-Globin was used as internal control. Comparing the sensitivity of four primers for HPV genotyping, that of GP1 primer was the highest , while the PGMY was the lowest. 99 women patients were initially analyzed by using GP1 primers for PCR and 25 samples out of them were HPV-negative by PCR. PCR/sequencing analysis from 74 cases showed that HPV-16 was most abundant (15%) and significant numbers of HPV-51, HPV-53, 56, 58 and 59(7% each) were also detected. These results were matched with the one from DNA chip analysis. Therefore, we concluded that GP1 PCR/direct sequencing method allows reliable identification HPV genotypes from HPV-infected samples.
저자
Jae-Hoon LEE [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
Hyun-Seok KIM [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
Keum Sook CHOI [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
Sun-Young BAEK [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
Young Lim KIM [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
Won-Sang HWANG [ Ewha University Women Biotech Life Science Institute ]
Haeng Suk CHOI [ Ewha University Women Biotech Life Science Institute ]
Seung-Chul KIM [ Ewha University Women Biotech Life Science Institute ]
Chan-Wha KIM [ Department of Life Sciences and Biotechnology School of Life Sciences and Biotechnology Korea university ]
Corresponding author
Sook-Jin HUR [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
Corresponding author
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동