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Genotyping Analysis of 74 Human Papillomavirus Types by a PCR/direct sequencing Method

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2007 춘계학술대회 및 국제심포지움 (2007.04)바로가기
  • 페이지
    pp.35-35
  • 저자
    Jae-Hoon LEE, Hyun-Seok KIM, Keum Sook CHOI, Sun-Young BAEK, Young Lim KIM, Won-Sang HWANG, Haeng Suk CHOI, Seung-Chul KIM, Chan-Wha KIM, Sook-Jin HUR
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A99782

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원문정보

초록

영어
The high-risk type HPV is the main etiological factor of cervical cancer, which is one of the leading causes of cancer deaths in women worldwide, Therefore, identification of HPV type is very important and necessary for the diagnosis and remedy of cervical cancer1-3). In this paper, we determined HPV genotypes from the patient samples, which were confirmed as Pap-smear positive for HPV infection. To identify genotypes, we performed PCR/sequencing analysis by
using the four different primer sets, PGMY, G4, G6 and GP1. At the same time, β-Globin was used as internal control. Comparing the sensitivity of four primers for HPV genotyping, that of GP1 primer was the highest , while the PGMY was the lowest. 99 women patients were initially analyzed by using GP1 primers for PCR and 25 samples out of them were HPV-negative by PCR.
PCR/sequencing analysis from 74 cases showed that HPV-16 was most abundant (15%) and significant numbers of HPV-51, HPV-53, 56, 58 and 59(7% each) were also detected. These results were matched with the one from DNA chip analysis. Therefore, we concluded that GP1 PCR/direct sequencing method allows reliable identification HPV genotypes from HPV-infected samples.

저자

  • Jae-Hoon LEE [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
  • Hyun-Seok KIM [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
  • Keum Sook CHOI [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
  • Sun-Young BAEK [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
  • Young Lim KIM [ Korea Food & Drug Administration Biological Diagnostic Products Team ]
  • Won-Sang HWANG [ Ewha University Women Biotech Life Science Institute ]
  • Haeng Suk CHOI [ Ewha University Women Biotech Life Science Institute ]
  • Seung-Chul KIM [ Ewha University Women Biotech Life Science Institute ]
  • Chan-Wha KIM [ Department of Life Sciences and Biotechnology School of Life Sciences and Biotechnology Korea university ] Corresponding author
  • Sook-Jin HUR [ Korea Food & Drug Administration Biological Diagnostic Products Team ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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