The response to phosphate starvation by PhoR-PhoB two-component regulatory system is controlled by other regulatory systems in Escherichia coli. In this study, the interactions among PhoB, PhoR, PhoU and CreC were investigated at combined transcript and protein levels using real-time PCR and fluorescence resonance energy transfer (FRET) analyses. The expressions of the phoB, phoR and creC genes are regulated depending on the concentration of environmental phosphate. The protein-protein interactions between PhoB-PhoR and -CreC are occurred depending on the concentration of environmental phosphate. These suggest that PhoU is a no direct regulator but CreC is an alternative regulator. These results showed that the detailed time- and phosphate concentration-dependent interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation of E. coli. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10309020000-03B5002-00000).Further supports by LG Chem Chair Professorship, Microsoft and IBM SUR program are appreciated.]
저자
Jong Hwan Baek [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center ]
Yeon Jae Kang [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center ]
Sang Yup Lee [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center, Department of Biosystems and Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
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