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Towards Conservation of Threatened Ceropegia Species Endemic to a Biodiversity Hotspot: In Vitro Microtuber Production and Proliferation, a Novel Strategy

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science 바로가기
  • 통권
    제24권 제2호 (2008.12)바로가기
  • 페이지
    pp.79-88
  • 저자
    Sagar Subhash Pandit, Aneeshkumar Nair, Dhiraj Dilip Naik
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A99688

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원문정보

초록

영어
Twenty-eight of 44 Indian Ceropegia species are endemic and their survival is threatened. As a step towards conservation, we implied in vitro methods for the sustainable propagule production in C. hirsuta, C. lawii, C. maccannii, C. oculata and C. sahyadrica. Effects of explant, growth regulators, sucrose and photoperiod were studied. High frequency microtuber production was achieved with the seedling-apical buds, grown on MS medium containing 4-6 mg l-1 BAP, 3-8% (w/v) sucrose, under continuous illumination. Each microtuber, when subcultured proliferated to form a cluster of secondary microtubers. Every primary and secondary microtuber bore at least one shoot-bud and a root primordium. Each tuber (formed with any of the significantly effective treatments) weighed more than 500 mg, enough to plant directly in non-sterilized soils. Microtubers could be produced and proliferated round the year. Proliferation could be solely attributed to in vitro procedures as these plants bear solitary tubers in vivo. Microtubers could be sprouted in vitro to prepare ready to pot plantlets. As, this novel method succeeded for all five species, though they belong to different eco-physiological backgrounds, we recommend its implementation in the conservation programs for a broader range of Ceropegia species, supported by other integrated strategies.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
  Morphology of microtubers
  Effect of explant
  Effect of PGRs on microtuberization
  Effect of sucrose on tuberization
  Effect of day length on tuberization
  Species specific response
  Sprouting of tubers
 DISCUSSION
 ACKNOWLEDGEMENTS
 LITERATURE CITED

키워드

Asclepiadaceae Endangered species Ex situ conservation Microtuberization Western ghats

저자

  • Sagar Subhash Pandit [ 1Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune-411008, MS, INDIA ] Corresponding author
  • Aneeshkumar Nair [ 1Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune-411008, MS, INDIA ]
  • Dhiraj Dilip Naik [ Department of Biology, West Virginia University, 53 Campus Drive, P.O. Box 6057, Morgantown, WV-26506, USA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2026
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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