Jatropha curcas L. is an oil bearing species with many uses and considerable economic potential as a biofuel crop. Salt stress effect on growth, ion accumulation, contents of protein, proline and antioxidant enzymes activity was determined in callus and seedling to understand the salt tolerance of the species. Exposure of callus and seedling to salt stress reduced growth in a concentration dependent manner. Under salt stress Na content increased significantly in both callus and seedling whereas, differential accumulation in the contents of K, Ca, and Mg was observed in callus and seedling. Soluble protein content differed significantly in callus as compared to seedling, however proline accumulation remained more or less constant with treatments. The proline concentration was ~2 to 3 times more in callus than in seedling. Salt stress induced qualitative and quantitative differences in superoxide dismutase (SOD; E.C. 1.15.1.1) and peroxidase (POX; E.C. 1.11.1.7) in callus and seedling. Salt induced changes of the recorded parameters were discussed in relation to salinity tolerance.
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ABSTRACT INTRODUCTION MATERIALS AND METHODS Callus culture and growth determination Germination percentage and seedling growth determination Determination of ion content Proline extraction and estimation Enzyme extractions and assays RESULTS Effect of salt on germination percentage and growthof callus and seedling Effect of salt on ion accumulation Effect of salt on protein and proline accumulation Effect of salt on the activity of antioxidant enzymes DISCUSSION ACKNOWLEDGEMENT REFERENCES
Nitish Kumar [ Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India ]
Meenakshi Kaur [ Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India ]
D.V.N. Sudheer Pamidimarri [ Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India ]
Girish Boricha [ Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India ]
Muppala P. Reddy [ Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India ]
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