Earticle

초록

영어

Acrylamide is present as a contaminant in heated food products, predominantly from the precursor asparagine. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. In order to assess the feasibility of microsatellite primers as markers for DNA damage, the study was conducted on common bean (Phaseolus vulgaris L.) exposed to different concentrations of acrylamide. Polymorphisms were abundant among plant samples treated with acrylamide in comparison to control (untreated one) tested with 4- tri-nucleotide, 2 tetra-nucleotide, and 3- dinucelotide primers. The primer (CCG)4 was the best tested primer to generate polymorphism between the DNA of plants treated or not by acrylamide. Polymorphisms became evident as the presence and absence of DNA fragments in treated samples compared with the untreated one. The highest number of DNA variation on ISSR patterns was observed at the micromollar concentrations of acrylamide. Acrylamide was able to induce DNA damage in non concentration-dependent manner with effectiveness at micromollar concentrations. This study demonstrated that ISSR markers can be highly reliable for identification of DNA damage induced by acrylamide.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Treatment and experimental design
  Electrophoresis of DNA and PCR products
  PCR amplification with ISSR primers
  Data collection
 RESULTS
  Optimization of ISSR
  Evaluation of ISSR to detect DNA damage in commonbean exposed to acryl amide
 DISCUSSION
 CONCLUSION
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Mohamed R. Enan [ Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, Giza, Egypt. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634