Isoflavone-O-β-D-Glucan extract from the submerged-liquid culture of Agaricus blazei Murill block Cell Cycle Progression and induces apoptosis in human breast MCF-7 cancer cells
Yun Ah Kim, Young Suk Kim, Seon Min Lee, Wook Jin Jang, M. A. Islam, Su Jeong Shim, Jeong Ok Kim, Cherl Woo Park, So Young Kim, Eun Ju Lee, Yeong Lae Ha.
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99674
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원문정보
초록
영어
The isoflavone-O-β-D-Glucan extract is a potent chemical component in the extract from the submerged-liquid culture of Agaricus blazei Murill. In the present study, the anti-tumor effect of isoflavone-O-β-D-Glucan extract was investigated using MCF-7 cells. MCF-7 cells were maintained with Dulbecco's Modified Eagle Medium/Ham's F-12 nutrient (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) at 37℃ in a humidified 5% CO2. For cell proliferation experiments, cells were seeded in 90 ㎜ dishes, treated with the various concentrations (0, 1, 5, 10, 15, 20, 25 ㎕) of the isoflavone-O-β-D-Glucan extract (10 ㎎/㎖) for over a period of 96 hr of incubation. The isoflavone-O-β-D-glucan inhibited the proliferation of cells in a dose-and time-dependent manner, which was mediated through up-regulation of p21/p53 and down-regulation of cyclin D. The results suggest that isoflavone-O-β-D-Glucan extract inhibited the proliferation of MCF-7 cells by induces apoptosis and block cell cycle progression in the G/S phase. This study was partly supported by the BK21 program of GNU.
저자
Yun Ah Kim [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
Young Suk Kim [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
Seon Min Lee [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
Wook Jin Jang [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
M. A. Islam [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
Su Jeong Shim [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
Jeong Ok Kim [ HK Biotech Co. Ltd. ]
Cherl Woo Park [ HK Biotech Co. Ltd. ]
So Young Kim [ Jinju International University ]
Eun Ju Lee [ Korea Food and Drug Administration, ]
Yeong Lae Ha. [ Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동