Cellulase activities assay for the enzyme produced from a filamentous fungus Trichoderma reesei strain QM9414 and Rut C-30 were performed. Reducing sugar released from cellulase hydrolysis was detected by dinitrosalicylic acid (DNS) method. The color developed from DNS was then measured by Digital Image Processing (DIP) technique in normal scale comparing the conventional spectrophotometric assay to the new DIP technique. Both spectrophotometric assay and DIP technique showed similar average cellulase activities with no statistically difference (P>0.05) among the 2 methods in normal scale. The cellulase activities of T. reesei QM9414 and Rut C-30 were measured, using mini-scale (5 times reduced) of DNS method comparing spectrophotometric assay to the DIP technique. The mini-scale of spectrophotometric assay and the DIP technique also displayed no difference of cellulase activities at confidence interval 95%. This study showed that the cellulase activity determination in the mini-scale by the DIP technique could rapidly be used for the determination with convenience and accuracy.
Tuenchai Kosakul [ Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University. Bangkok 10330, Thailand. ]
Kanyawan Satirwut [ Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University. Bangkok 10330, Thailand. ]
Sehanat Prasongsuk [ Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University. Bangkok 10330, Thailand. ]
Arun Hansuebsai [ Department of Image and Printing Technology, Faculty of Science, Chulalongkorn University. Bangkok 10330, Thailand. ]
Hunsa Punnapayak [ Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University. Bangkok 10330, Thailand. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동