You-Jung Lee, Hye-Jin Kim, Chung-Han Chung, Jin-Woo Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99566
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
The gene coding for carboxymethylcellulase (CMCase) of B. amyoliquefaciens DL-3 was isolated and cloned in E. coli JM109F. Rice bran and tryptone were chosen as carbon and nitrogen sources for production of CMCase by E. coli JM109F, respectively. Optimal conditions such as concentrations of carbon sources and nitrogen sources, initial pH of medium, and temperature for production of CMCase were investigated using L25(5)3 - orthogonal array method with five distinct levels and three factors. The highest production of CMCase was 1,314.2 U/ml from 5.0% (w/v) rice bran and 0.1% (w/v) tryptone under initial pH of 7.5 and 37℃. Optimal conditions of salts for production of CMCase were also investigated using L25(5)4. The highest production of CMCase E. coli JM109F was 1,514.6 U/ml with 0.5% (w/v) K2HPO4, 0.025% (w/v) NaCl, 0.04% MgSO4․7H2O and 0.24%(w/v) (NH4)2SO4.
저자
You-Jung Lee [ Department of Medical Bioscience, Graduate School of Dong-A University, BK21 Bio-Silver Group, Dong-A University ]
Hye-Jin Kim [ Department of Medical Bioscience, Graduate School of Dong-A University, BK21 Bio-Silver Group, Dong-A University ]
Chung-Han Chung [ 2Department of Biotechnology, BK21 Bio-Silver Group, Dong-A University ]
Jin-Woo Lee [ 2Department of Biotechnology, BK21 Bio-Silver Group, Dong-A University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동