It has been reported that Streptomyces avermitilis is able to produce a number of potent anthelmintic and insecticidal agents known as the avermectins. Among those compounds, avermectin B1a has the most effective antiparasitic activity, and has been successfully commercialized as a powerful agricultural pesticide and antiparasitic agent. Mutant strain of S. avermitilis ATTC 31271(AJ-7) was obtained via random mutation using NTG (3 mg/ml, 60 minutes). For further improvement of avermectin B1a production from AJ-7, optimization of current avermectin B1a production medium was studied. Result of medium optimization was showed productivity of 475.6 mg/L which is about 300 times greater than before medium optimization. Cobalt and vitamin B12 are believed to be the most critical elements in secondary metabolism. Furthermore attempts were made to increase avermectin B1a production using Co2+ and Vitamin B12. As a result, avermectin B1a production decreased with higher concentration of CoCl2 and vitamin B12. The results indicated that two substances are not required for increasing production of avermectin B1a in AJ-7. Using these results currently, fermentation using bioreactor to achieve scale up is underway.
저자
Eun-Young Jeong [ Department of Molecular Science and Technology, Ajou university ]
In-Woo Kim [ Department of Molecular Science and Technology, Ajou university ]
Yeon-Woo Ryu [ Department of Molecular Science and Technology, Ajou university ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동