Ji-Hye AN, Byoung Seung JEON, Dong-Hyun NAM, Min-Kyu OH, Yong-Hwan KIM, Byoung-In SANG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99404
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원문정보
초록
영어
Covalent attachment, aggregate coat ing or entrapment of enzymes with nanof ibers yields highly act ive and stable systems for the applicat ions in the various f ields such ac bioconversion, biomedicals, and biosensors. In this study, we compared the stabilit ies of enzymes, which were immobilized by several nano-based biosilif icat ion. Glutathione Stransferase (GST) as the model enzyme are used, and GST and R5 tagged GST gene was amplif ied by PCR and inserted into the pET-28 a(+) vector (Novagen). It was expressed in E.coli BL21, and then purif ied with aff inity chromatography. GST was immobilized into the nanof iber consisted of polystyrene (PS) and poly (styrene-co-maleic anhydride) (PSMA). R5 catalyzed the format ion of silica nanopart icles (about 200 nm in diameter) with GST retained its act ivity.
저자
Ji-Hye AN [ Korea Inst itute of Science and Technology ]
Byoung Seung JEON [ Korea Inst itute of Science and Technology ]
Dong-Hyun NAM [ Department of Chemical Engineering, Kwangwoon University ]
Min-Kyu OH [ Department of Chemical & Biological Engineering, KOREA University ]
Yong-Hwan KIM [ Department of Chemical Engineering, Kwangwoon University ]
Byoung-In SANG [ Korea Inst itute of Science and Technology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동