Ohseok KWON, Seonyeong BAK, Miseop CHOI, Keunjun KIM, Kyungju HAN, Youngjun SEO, Gihyun KIM, Kyungtak NAM, Jusun NAM, Youngeun KIM, Gisuk JUNG, Kyungmin BAEK, Sookyung SUH, Seunghwa HONG
언어
영어(ENG)
URL
https://www.earticle.net/Article/A99281
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원문정보
초록
영어
Erythropoietin (EPO) is a glycoprotein hormone primarily synthesized in kidney and regulates mammalian erythropoiesis1). In this study, N-glycans of recombinant human erythropoietins (rhEPO) expressed in insect cells were analyzed to investigate the heterogeneity according to different cell culture methods. rhEPO was designed to have mouse derived IgM signal sequence for secretion out of the cell and IgG binding domain of protein A at N-terminal for efficient purification. Although insect cells are usually cultured at 27℃, various culture temperatures such as 24℃, 30℃, 33℃, 37℃ were tested. 24℃ showed highest productivity for rhEPO and the productivity was decreased according as the culture temperature increased. Where as the productivity was increased at low temperature, N-glycan profile of rhEPO from 24℃ culture was similar with that of 27℃ culture.
저자
Ohseok KWON [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Seonyeong BAK [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Miseop CHOI [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Keunjun KIM [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Kyungju HAN [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Youngjun SEO [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Gihyun KIM [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Kyungtak NAM [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Jusun NAM [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Youngeun KIM [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Gisuk JUNG [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Kyungmin BAEK [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Sookyung SUH [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
Seunghwa HONG [ Recombinant Products Division, Biologics Bureau, Korea Food & Drug Administration ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동