Earticle

초록

영어

To support and enhance the in vitro growth and activity, the effects of various chemical
factors, such as growth factors, cytokines, and hormones on cellular metabolic responses
were investigated. It was recently reported that mechanical tension also play a central role
in the physiology of a wide variety of tissues.
In this study, we evaluate the effects of mechanical tension on the proliferation and ECM
production of human dental pulp stem cells (DPSCs) using a flexwell system that imposed
cyclic mechanical tension. This result that DPSC proliferation under tension varied with
culture time. Mechanical tension increase the of lactate dehydrogenase during culture.
RT-PCR mechanical tension increased collagen and osteopontin expression and decreased α
-SMA expression. FACS analysis showed that CD105 expression did not change in all
groups but CD 90 expression decreased at 8 % strain. In conclusion, that appropriate level
of mechanical tension serve as a potent positive modulator of proliferation, differentiation
and ECM production DPSCs.

저자

• Young-KwonSeo [ Dongguk University Research Institute of Biotechnology, Dongguk University ]
• Hee-HoonYoon [ Dongguk University Research Institute of Biotechnology, Dongguk University ]
• Kye-Yong Song [ Department of Pathology, School of Medicine, Chung-Ang University ]
• Jung-Keug Park [ Dongguk University Research Institute of Biotechnology, Dongguk University, Department of Chemical and Biochemical Engineering, Dongguk University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576