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Application of Serum-free Conditioned M edia from M ixed Cells for the Cultivation of M SCs

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2008 추계학술대회 및 국제심포지움 (2008.10)바로가기
  • 페이지
    pp.154-154
  • 저자
    Bu-Y oung Park, Yong-Soo Choi, Jong-Il Kim, Ji-Hae Song, Hyung-Jin Jeon, Young-Joo Jeon, Sung-Hun Choi, Dong-Il Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A99268

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원문정보

초록

영어
Not only have mesenchymal stem cells (MSCs) multilineage potency, but also provide a supportive cellular microenvironment. The cellular capabilities of expansion and differentiation are known to be strongly affected by environment and media. For the serum-free cultivation of MSCs, the effect of serum-free conditioned media (CM) from mixed cells on the expansion of
bone marrow (BM)-MSCs was investigated. We substituted fetal bovine serum (FBS) by serum-free media (SFM) for the elimination of possible contaminations and provided CM for their microenvironments. The mixed cells were composed of umbilical cord blood-MSCs, BM-MSCs,
periosteum-derived progenitor cells and fibroblasts. Cell viability, growth kinetics and MSCs specific surface makers were compared between serum-free CM and culture media containing 10% FBS . Although the growth of MSCs in serum-free CM was not higher than those in culture media containing 10% FBS, surface makers and differentiation capability of MSC were not significantly different. Based on these results, serum-free CM from mixed cells could be used for cultivation of MSCs and their differentiation capacity was maintained.

저자

  • Bu-Y oung Park [ Department of Biological Engineering, Inha University ]
  • Yong-Soo Choi [ Department of Biological Engineering, Inha University ]
  • Jong-Il Kim [ Department of Biological Engineering, Inha University ]
  • Ji-Hae Song [ Department of Biological Engineering, Inha University ]
  • Hyung-Jin Jeon [ Department of Biological Engineering, Inha University ]
  • Young-Joo Jeon [ Department of Biological Engineering, Inha University ]
  • Sung-Hun Choi [ Department of Biological Engineering, Inha University ]
  • Dong-Il Kim [ Department of Biological Engineering, Inha University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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    피인용수 : 0(자료제공 : 네이버학술정보)

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