Earticle

초록

영어

A bacterial strain, MBBL 2-9, displaying potent antimicrobial activity against Grampositive bacteria, including Staphylococcus aureus, was isolated from vaginal microbiota of healthy woman. Based on its morphological properties and 16S rRNA sequence analysis, the bacterium was identified as Staphylococcus hominis. A new bacteriocin named as hominicin was purified by chloroform extraction, cation-exchange column chromatography and reverse-phase HPLC. Hominicin was heat stable up to 121°C, 15 min and active under conditions of acid/alkali (pH 2.0 to 9.0). Antimicrobial activity was lost after treatment with proteinase K, but trypsin, chymotrypsin, pepsin, and lipase had no effect on hominicin activity. Also, hominicin not showed cytotoxicity on blood agar plate after incubation for 24 h. The molecular mass of purified hominicin as determined by mass spectrometry was 2,038 Da. MS/MS results suggested that
hominicin consist of peptidic and non-peptidic moiety. De novo sequencing of peptidic moiety revealed that Ala-Ala-Val-Leu/Ile-Ala-Gly and no identity with previously reported bacteriocins. Plasmid curing result indicated that a plasmid (about 5 kb in size), designated as pSH29, seemed to be responsible for hominicin production. This present study is the first report of a novel bacteriocin, hominicin, produced by S. hominis that has potential for use as an alternative antimicrobial agent for the treatment of infection with Gram-positive bacteria. This work was supported by a grant (20080401034009) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

저자

• Pyoung Il Kim [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University ]
• Hwang-Soo Joo [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University ]
• Eun-Mi Kim [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University ]
• Changmin Sung [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University ]
• Hae-Min [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University ]
• Byung-Gee Kim [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576