Earticle

초록

영어

G-protein coupled receptors (GPCRs), a family of integral membrane proteins with seven
transmembrane helices, are difficult to be overexpressed, solubilized and purified, especially from bacterial cell, because of their complicated structures and strong hydrophobicity. Before applying to chromatographic purification method, solubilization of GPCR expressed as inclusion body is critical step for GPCR production. In this work, a human olfactory receptor, hOR17-40, a family of GPCR, was overexpressed at high-level in E. coli mainly as insoluble form of inclusion body. Expressed human olfactory receptor was difficult to be solublilized by many detergents and chaotropes and sarcosyl and SDS was found to be suitable for effiient solubilization. Sarcosyl showed better solubilization ability than SDS and was thought to be the most suitable detergent for solubilization.
Solubilization of inclusion body was performed with different concentration of sarcosyl and
solubilized protein quantities were analyzed with immunoblotting and total protein assay. 5% sarcosyl was determined as optimal concentration for inclusion body solublization of the human olfactory receptor. After solubilization of GPCR, further chromatographic purification method can be applied to produce GPCR that is for being used in the fields of drug discovery and development of biosensor.

키워드

저자

• Hyun Seok Song [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea ]
• Sang Hun Lee [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea ]
• Eun Hae Oh [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea ]
• Jong Hyun Lim [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea ]
• Tai Hyun Park [ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576