Earticle

초록

영어

Oxanine (Oxa), generated as one of the major products from guanine by nitrosative oxidation, has been studied as a mutagenic lesion [1]. Here, Oxa was focused in terms of its unique property to react with NH2 group. Especially, Oxa having an O-acylisourea structure was explored to see if its reactivity with amino group is useful in DNA microarray fabrication. The nucleotide unit of oxanine (Oxa-N) was incorporated into the 5'-end of probe DNA by the chemical synthesis method [2] and the synthetic probe DNA was spotted onto the NH2-functionalized glass slide. It was found that the Oxa-DNA probe was immobilized on the glass surface by one-pot reaction, producing the high-efficiency of the target hybridization. Thanks to activation-free reactivity of Oxa, the post-spotting treatment can be performed under the mild conditions (at 25 or 42C), which could be favorable for maintaining effective hybridization
performance of the immobilized DNA probe. Further, it has been determined under the mild conditions that the humidity and time of the post-spotting treatment, pH of the spotting solution and the synergistic effects with UV-irradiation largely contribute to the desired immobilization and resulting target recognition [3]. Immobilization of DNA oligomer by use of Oxa-N on the NH2-functionalized surface without any activation step can be used as one of the advanced methods for generating DNA-conjugated solid surface.

저자

• Seung Pil Pack [ Department of Biotechnology and Bioinformatics, Korea University ]
• Keisuke Makino [ Institute of Advanced Energy, Kyoto University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576